二硫键连接肽的检测与片段化改进

Maes Evelyne, Clerens Stefan, Dyer Jolon M, Deb-Choudhury Santanu

Food & Bio-Based Products, AgResearch Ltd., 1365 Springs Road, Lincoln 7674, New Zealand.

Biomolecular Interaction Centre, University of Canterbury, Christchurch 8140, New Zealand.

Methods Protoc. 2018 Sep 3;1(3):33. doi: 10.3390/mps1030033.

Characterisation of peptides containing intact disulphide bonds (DSBs) via mass spectrometry is challenging. Our study demonstrates that the addition of aniline to alpha-cyano-4-hydroxycinnamic acid improves detection and fragmentation of complex DSB peptides by matrix-assisted laser desorption/ionization, tandem time-of-flight mass spectrometry (MALDI-TOF-TOF MS). This improved assignment will be a significant new tool when a simple screening to confirm the DSB existence is required.

通过质谱对含有完整二硫键（DSB）的肽进行表征具有挑战性。我们的研究表明，在α-氰基-4-羟基肉桂酸中添加苯胺可通过基质辅助激光解吸/电离串联飞行时间质谱（MALDI-TOF-TOF MS）改善复杂DSB肽的检测和碎片化。当需要进行简单筛选以确认DSB的存在时，这种改进的分析方法将成为一种重要的新工具。