Bieglmayer C, Jettmar W, Adamiker D, Spona J
Endocrinol Exp. 1978 Jun;12(2):89-101.
Levels of estrogen binding proteins in the cytosol of hypothalamic and pituitary tissue of male rats at different ages were estimated. Two classes of estrogen binding sites were detected in 13 and 20 days old animals. The number of high affinity estrogen binding sites (K = ca. 2 x 10(-10) mol) of the hypothalamus was slightly larger in 13 days old animals, then decreased to constant levels at the age of 20 days and no changes were observed thereafter. Levels of low affinity binding sites of the hypothalamus (K = ca. 5 x 10(-9) mol) decreased with age and were no longer detectable in 30 days old animals. Similarly, a decrease of low affinity estrogen binding sites with age was recorded for pituitary cytosol samples and this class of receptor sites was no longer observable in 30 days old male rat. At 13 days high levels of high affinity 17 beta-estradiol binding protein was registered and minimal numbers of binding sites were found at 20, 30 and 40 days. Thereafter, the levels remained constant until 100 days and were similar to values observed in the 13 days old male animals. Apparent affinity constants of two classes of binding sites in hypothalamus and pituitary were similar...
对不同年龄雄性大鼠下丘脑和垂体组织细胞质中雌激素结合蛋白的水平进行了评估。在13日龄和20日龄的动物中检测到两类雌激素结合位点。13日龄动物下丘脑高亲和力雌激素结合位点（K = 约2×10⁻¹⁰摩尔）的数量略多，然后在20日龄时降至恒定水平，此后未观察到变化。下丘脑低亲和力结合位点（K = 约5×10⁻⁹摩尔）的水平随年龄下降，在30日龄动物中不再可检测到。同样，垂体细胞质样品中低亲和力雌激素结合位点也随年龄下降，在30日龄雄性大鼠中这类受体位点不再可观察到。在13日龄时记录到高亲和力17β-雌二醇结合蛋白水平较高，在20、30和40日龄时发现结合位点数量最少。此后，这些水平一直保持恒定直至100日龄，且与13日龄雄性动物中观察到的值相似。下丘脑和垂体中两类结合位点的表观亲和常数相似……
