Klemme J C, Mukhtar H, Elmets C A
Department of Dermatology, University Hospitals of Cleveland, Ohio 44106.
Cancer Res. 1987 Nov 15;47(22):6074-8.
The potential for allergic contact sensitization to two polyaromatic hydrocarbons, 7,12-dimethylbenz(a)anthracene and benzo(a)pyrene, was investigated in C3H/HeN mice. For each agent, contact hypersensitivity was achieved by applying a 100-micrograms dose to the shaved abdomen. An ear swelling response was observed following application of 20 micrograms of the sensitizing dose to the ear dorsum, whereas unsensitized animals or animals sensitized to the alternate agent did not develop ear swelling. Histological sections of DMBA-challenged ears revealed edema with a marked dermal mononuclear infiltrate. Following adoptive transfer of draining lymph node cells of DMBA-sensitized mice to naive syngeneic mice, ear challenge of recipient mice with the same agent resulted in an ear swelling response. This demonstration of in vivo cell-mediated immunological reactivity to polyaromatic hydrocarbon carcinogens indicates that the immune system can interact with and, potentially, modify the course of chemical carcinogens at a time prior to morphological changes or development of tumor-specific transplantation antigens.
在C3H/HeN小鼠中研究了对两种多环芳烃7,12 - 二甲基苯并(a)蒽和苯并(a)芘发生过敏性接触致敏的可能性。对于每种试剂，通过在剃毛的腹部涂抹100微克剂量来实现接触性超敏反应。在向耳背涂抹20微克致敏剂量后观察到耳部肿胀反应，而未致敏的动物或对另一种试剂致敏的动物未出现耳部肿胀。经DMBA激发的耳部组织学切片显示有水肿，并伴有明显的真皮单核细胞浸润。将DMBA致敏小鼠的引流淋巴结细胞过继转移至同基因的未致敏小鼠后，用相同试剂对受体小鼠进行耳部激发会导致耳部肿胀反应。这种对多环芳烃致癌物的体内细胞介导免疫反应性的证明表明，免疫系统可以在形态学变化或肿瘤特异性移植抗原出现之前与化学致癌物相互作用，并有可能改变其进程。
