Microbiology & Immunology Section, Animal Health Department, Faculty of Veterinary Medicine, University of León, Spain.
Porcine Immunology Laboratory, Biotechnology Department, INIA, Madrid, Spain.
Comp Immunol Microbiol Infect Dis. 2019 Jun;64:31-39. doi: 10.1016/j.cimid.2019.02.001. Epub 2019 Feb 8.
TLRs, Siglecs and CD163 are cell surface receptors that play an important role in immune response and sepsis. The objective of this study was to assess changes in the expression levels of several of these receptors (TLR2, TLR4, CD163, Siglec-1, Siglec-3, Siglec-5 and Siglec-10) on the surface of peripheral blood mononuclear cells from pigs with sepsis caused by Haemophilus parasuis. Flow cytometry was employed to analyze samples from an experimental infection and from cell cultures. A significant increase in CD163, TLR2 and Siglec-3 expression during infection was seen. However, in vitro exposure of peripheral blood monocytes to bacteria or sera from infected pigs did not increase the expression of these receptors. These changes may be due to recruitment of monocytes into the blood compartment in response to H. parasuis-induced sepsis.
TLRs、Siglecs 和 CD163 是细胞表面受体，它们在免疫反应和败血症中起着重要作用。本研究的目的是评估几种这些受体（TLR2、TLR4、CD163、Siglec-1、Siglec-3、Siglec-5 和 Siglec-10）在由副猪嗜血杆菌引起的败血症的猪外周血单核细胞表面的表达水平的变化。流式细胞术用于分析来自实验感染和细胞培养的样本。在感染过程中观察到 CD163、TLR2 和 Siglec-3 的表达显著增加。然而，体外将外周血单核细胞暴露于细菌或来自感染猪的血清并没有增加这些受体的表达。这些变化可能是由于副猪嗜血杆菌诱导的败血症导致单核细胞募集到血液中。
