Department of Biotechnology, Savitribai Phule Pune University, Pune 411007, India.
Department of Biotechnology, Savitribai Phule Pune University, Pune 411007, India.
Food Chem. 2019 Oct 15;295:403-411. doi: 10.1016/j.foodchem.2019.05.096. Epub 2019 May 14.
The aim of this study was to examine the variations in sugar content and identify the polymorphism in vacuolar invertase inhibitor (INH2) gene from Indian non-processing (Kufri Jyoti, Kufri Pukhraj and PU1) and exotic processing (Atlantic and Frito Lay-1533) potato cultivars. Upon cold storage (4 °C) processing cultivars maintained low reducing sugars as compared to non-processing cultivars. Sequencing of the INH2 gene identified four alleles of which three identified as novel alleles. A total twelve SNPs resulted in silent mutations, with five conferring the amino acid substitutions. Phylogenetic analysis suggests a highly conserved nature of the INH2 gene. The 3D predicted structures generated for all the alleles revealed slight variations in the orientation of the helices (α1-3) in N-terminal region. Sequence polymorphism observed in INH2 alleles in processing and non-processing potato cultivars can be correlated with the observed variations in the sugar content suggesting a possible role in cold-induced sweetening.
本研究旨在探讨糖含量的变化，并从印度非加工（Kufri Jyoti、Kufri Pukhraj 和 PU1）和外来加工（Atlantic 和 Frito Lay-1533）马铃薯品种中鉴定液泡转化酶抑制剂（INH2）基因的多态性。冷储存（4°C）后，与非加工品种相比，加工品种的还原糖含量较低。对 INH2 基因进行测序，共鉴定出四个等位基因，其中三个为新等位基因。总共发现了 12 个导致沉默突变的单核苷酸多态性，其中 5 个导致氨基酸取代。系统发育分析表明 INH2 基因具有高度保守的性质。所有等位基因的 3D 预测结构显示，N 端区域的螺旋（α1-3）的取向略有变化。在加工和非加工马铃薯品种中观察到 INH2 等位基因的序列多态性可能与观察到的糖含量变化相关，表明其在冷诱导变甜中可能发挥作用。
