Clinical Immunogenetics Laboratory, Seattle Cancer Care Alliance, Seattle, WA, USA; University of Washington, Seattle, WA, USA.
Clinical Immunogenetics Laboratory, Seattle Cancer Care Alliance, Seattle, WA, USA.
Hum Immunol. 2019 Oct;80(10):828-833. doi: 10.1016/j.humimm.2019.05.010. Epub 2019 Jun 5.
Mismatching of an unrelated donor against a high-expression HLA-DPB1 recipient allele is associated with a high risk of graft-versus-host disease and mortality. The Seattle Cancer Care Alliance (SCCA) and Fred Hutchinson Cancer Research Center transplant program employs an algorithm to match for HLA-A, B, C, DRB1, DQB1 and DPB1 alleles (12/12) and to avoid, whenever possible, donor mismatching against a recipient high-expression HLA-DPB1 allele. HLA-DPB1 expression is associated with the rs9277534 A/G polymorphism located in the 3'UTR of the HLA-DPB1 gene. Next generation sequencing of HLA-DPB1 using the Illumina TruSight HLA V2 Sequencing Panel and Conexio Assign software analyses provides information on rs9277534 variants without the need for any additional SNP testing. Here we present the molecular location of rs9277534 in NGS data and discuss the challenges to resolve HLA-DPB1 ambiguities.
不相关供体与高表达 HLA-DPB1 受体等位基因不匹配与移植物抗宿主病和死亡率的高风险相关。西雅图癌症护理联盟（SCCA）和弗雷德·哈钦森癌症研究中心移植项目采用一种算法来匹配 HLA-A、B、C、DRB1、DQB1 和 DPB1 等位基因（12/12），并尽可能避免供体与高表达 HLA-DPB1 受体等位基因不匹配。HLA-DPB1 的表达与位于 HLA-DPB1 基因 3'UTR 中的 rs9277534 A/G 多态性相关。使用 Illumina TruSight HLA V2 测序面板和 Conexio Assign 软件分析的 HLA-DPB1 下一代测序提供了有关 rs9277534 变体的信息，而无需进行任何额外的 SNP 测试。在这里，我们展示了 rs9277534 在 NGS 数据中的分子位置，并讨论了解决 HLA-DPB1 歧义的挑战。
