Makhoul N, Merchav S, Tatarsky I, Naot Y
Department of Immunology, Faculty of Medicine, Technion-Israel Institute of Technology, Haifa.
Isr J Med Sci. 1987 May;23(5):480-4.
Mitogenic doses of membranes purified from Mycoplasma pneumoniae as well as concanavalin A (ConA) were tested for their ability to induce production of interleukin-2 (IL-2) and granulocyte-macrophage colony-stimulating activity (GM-CSA) by human peripheral blood mononuclear cells. Unlike ConA, M. pneumoniae exhibited a significantly lower mitogenic effect and lacked the ability to induce production of IL-2. On the other hand, peak levels of GM-CSA produced in the presence of M. pneumoniae and detected in colony assays of human target marrow cells were approximately 80% of those produced in the presence of ConA. Whereas ConA induced a similar time-related effect upon [3H]thymidine uptake and GM-CSA production, maximal GM-CSA production preceded the peak proliferative response to M. pneumoniae, thereby indicating that M. pneumoniae-induced production of GM-CSA is not quantitatively correlated with DNA synthesis. These findings may contribute to the understanding of the inflammatory manifestations induced by this organism.
对从肺炎支原体中纯化的膜以及伴刀豆球蛋白A（ConA）的促有丝分裂剂量进行了测试，以检测它们诱导人外周血单个核细胞产生白细胞介素-2（IL-2）和粒细胞-巨噬细胞集落刺激活性（GM-CSA）的能力。与ConA不同，肺炎支原体表现出显著较低的促有丝分裂作用，并且缺乏诱导IL-2产生的能力。另一方面，在肺炎支原体存在下产生并在人靶骨髓细胞集落测定中检测到的GM-CSA峰值水平约为ConA存在下产生水平的80%。虽然ConA对[3H]胸苷摄取和GM-CSA产生诱导了类似的时间相关效应，但GM-CSA的最大产生先于对肺炎支原体的增殖反应峰值，从而表明肺炎支原体诱导的GM-CSA产生与DNA合成在数量上不相关。这些发现可能有助于理解该生物体诱导的炎症表现。
