非热等离子体对多能基质细胞增殖活性及与组织工程构建体所用支架黏附的影响
Effect of Non-Thermal Plasma on Proliferative Activity and Adhesion of Multipotent Stromal Cells to Scaffolds Developed for Tissue-Engineered Constructs.
作者信息
Chailakhyan R K, Grosheva A G, Gerasimov Yu V, Vorob'eva N N, Ermolaeva S A, Sysolyatina E V, Kazakova M V, Akishev Yu S, Petryakov A V, Sidoruk K V, Burdukovskii V F, Timashev P S
机构信息
N. F. Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia.
Institute of Photonic Technologies, Federal Research Center for Crystallography and Photonics, Russian Academy of Sciences, Moscow, Russia.
出版信息
Bull Exp Biol Med. 2019 May;167(1):182-188. doi: 10.1007/s10517-019-04486-0. Epub 2019 Jun 8.
We studied the effect of non-thermal argon plasma on proliferative activity of bone marrow multipotent stromal cells in vitro. Treatment of stromal cell suspension with pure argon did not affect their proliferation. The cells treated with non-thermal argon plasma and explanted in the treatment medium demonstrated growth inhibition by 30-40% in comparison with the control. Multipotent stromal cells treated with plasma and after centrifugation explanted in normal medium within 12 min demonstrated accelerated growth. The total cell growth from the pellet and supernatant significantly exceeded the control values. We also analyzed adhesion and proliferative activity of multipotent stromal cells treated with non-thermal plasma on bioresorbable carriers. The cells adhered and proliferated on all types of studied samples. Adhesion properties of scaffolds differed. Caprolactone was found to be the most suitable material for adhesion and proliferation of multipotent stromal cells.
我们研究了非热氩等离子体对体外骨髓多能基质细胞增殖活性的影响。用纯氩处理基质细胞悬液不影响其增殖。用非热氩等离子体处理并接种于处理培养基中的细胞与对照组相比，生长受到30 - 40%的抑制。经等离子体处理并在12分钟内离心后接种于正常培养基中的多能基质细胞显示生长加速。来自沉淀和上清液的总细胞生长显著超过对照值。我们还分析了用非热等离子体处理的多能基质细胞在生物可吸收载体上的黏附及增殖活性。细胞在所有类型的研究样本上均能黏附并增殖。支架的黏附特性有所不同。已发现己内酯是多能基质细胞黏附及增殖的最合适材料。
Zotero 插件