Vaikkath Dhanesh, Anitha Rakhi, Sumathy Babitha, Nair Prabha D
Division of Tissue Engineering and Regeneration Technologies, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, India.
Colloids Surf B Biointerfaces. 2016 May 1;141:112-119. doi: 10.1016/j.colsurfb.2015.12.041. Epub 2015 Dec 28.
A number of biodegradable and bioresorbable materials, as well as scaffold designs, have been experimentally and/or clinically studied for tissue engineering of diverse tissue types. Cell-material responses are strongly dependent on the properties of the scaffold material. In this study, scaffolds based on polycaprolactone (PCL) and PCL blended with a triblock copolymer, Polycaprolactone-polytetrahydrofuran-polycaprolactone (PCL-PTHF-PCL) at different ratios were fabricated by electrospinning. Blending and electrospinning of the triblock copolymer with PCL generated a super hydrophilic scaffold, the mechanical and biological properties of which varied with the concentration of the triblock copolymer. The hydrophilicity of the electrospun scaffolds was determined by measurement of water-air contact angle. Cellular response to the electrospun scaffolds was studied by seeding two types of cells, L929 fibroblast cell line and rat mesenchymal stem cells (RMSC). We observed that the super hydrophilicity of the material did not prevent cell adhesion, while the cell proliferation was low or negligible for scaffolds containing higher amount of PCL-PTHF-PCL. Chondrogenic differentiation of RMSC was found to be better on the PCL blend containing 10% (w/v) of PCL-PTHF-PCL than the bare PCL. Our studies indicate that the cellular response is dependent on the biomaterial composition and highlight the importance of tailoring the scaffold properties for applications in tissue engineering and regenerative medicine.
多种可生物降解和生物可吸收材料以及支架设计已在实验和/或临床层面针对不同组织类型的组织工程进行了研究。细胞与材料的相互作用很大程度上取决于支架材料的特性。在本研究中，通过静电纺丝制备了基于聚己内酯（PCL）以及不同比例的PCL与三嵌段共聚物聚己内酯-聚四氢呋喃-聚己内酯（PCL-PTHF-PCL）共混的支架。三嵌段共聚物与PCL的共混和静电纺丝产生了一种超亲水支架，其机械性能和生物学性能随三嵌段共聚物浓度的变化而变化。通过测量水-空气接触角来测定静电纺丝支架的亲水性。通过接种两种细胞，即L929成纤维细胞系和大鼠间充质干细胞（RMSC），研究了细胞对静电纺丝支架的反应。我们观察到材料的超亲水性并不妨碍细胞黏附，而对于含有较高量PCL-PTHF-PCL的支架，细胞增殖较低或可忽略不计。发现RMSC在含有10%（w/v）PCL-PTHF-PCL的PCL共混物上的软骨分化比纯PCL更好。我们的研究表明细胞反应取决于生物材料的组成，并强调了为组织工程和再生医学应用定制支架特性的重要性。
