一种用于甲氨蝶呤和其他叶酸拮抗剂的简单放射性测定法。
A simple radiometric assay for methotrexate and other folate antagonists.
作者信息
Hayman R J, Fong H, Van der Weyden M B
出版信息
J Lab Clin Med. 1979 Mar;93(3):480-4.
A rapid radiometric assay for the folate antagonist MTX, based on the inhibition of chicken liver dihydrofolate reductase activity, has been developed. In the system the inhibition by MTX of the reduction of tritiated FH2 is standardized and used to quantitate this antagonist in plasma of subjects receiving high-dose MTX therapy. The assay permits the detection of plasma MTX levels to 5 X 10(-9)M with a coefficient of variation of 15%. The predominating circulating folate, 5 methyltetrahydrofolate, or the rescue agent leucovorin (5 formyltetrahydrofolate) do not significantly interfere with the assay at concentrations attainable with current rescue programs. Analyses of multiple plasma samples with the present assay show close agreement with the competitive protein-binding assay.
已开发出一种基于抑制鸡肝二氢叶酸还原酶活性的快速放射测定法，用于检测叶酸拮抗剂甲氨蝶呤（MTX）。在该系统中，MTX对氚化FH2还原的抑制作用已标准化，并用于定量接受高剂量MTX治疗患者血浆中的这种拮抗剂。该测定法可检测至5×10（-9）M的血浆MTX水平，变异系数为15%。在当前救援方案所能达到的浓度下，主要循环叶酸5-甲基四氢叶酸或救援剂亚叶酸钙（5-甲酰四氢叶酸）不会对该测定法产生显著干扰。用本测定法对多个血浆样本进行分析，结果与竞争性蛋白结合测定法密切相符。
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