骨髓来源及胎儿来源巨噬细胞吞噬活性的定量与定性特征分析
Quantitative and Qualitative Characterization of Phagocytic Activity of Macrophages of Bone Marrow and Fetal Origin.
作者信息
Lokhonina A V, Makarov A V, Elchaninov A V, Arutyunyan I V, Shmakova T V, Grinberg M V, Usman N Yu, Surovtsev V V, Chernikov V P, Fatkhudinov T Kh
机构信息
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
Peoples' Friendship University of Russia, Moscow, Russia.
出版信息
Bull Exp Biol Med. 2019 May;167(1):154-158. doi: 10.1007/s10517-019-04481-5. Epub 2019 Jun 10.
We compared phagocytic activity of macrophages of monocyte origin and Kupffer cells under the influence of M1 and M2 inducers and without activation. Cultures of monocyte-derived macrophages and Kupffer cells were characterized by intensive expression of CD68 that was not affected by activation factors. At the same time, these cultures demonstrated different dynamics of phagocytic activity. Monocyte-derived macrophages initially had more pronounced absorption capacity that gradually increased during the experiment. Kupffer cells were characterized by abrupt fluctuations of phagocytic activity: sharp growth and rapid saturation. Despite these differences, the endosomes produced by monocyte-derived macrophages and Kupffer cells had similar degrees of maturity.
我们比较了单核细胞来源的巨噬细胞和库普弗细胞在M1和M2诱导剂影响下以及未激活状态下的吞噬活性。单核细胞衍生的巨噬细胞和库普弗细胞培养物的特征是CD68表达强烈，且不受激活因子影响。同时，这些培养物表现出不同的吞噬活性动态。单核细胞衍生的巨噬细胞最初具有更明显的吸收能力，在实验过程中逐渐增加。库普弗细胞的特征是吞噬活性急剧波动：急剧增长和迅速饱和。尽管存在这些差异，但单核细胞衍生的巨噬细胞和库普弗细胞产生的内体具有相似的成熟度。
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