Meng Xu, Jian-Hai Yin, Sheng-Kui Cao, Jian-Ping Cao, Xiao-Fan Zhang, Yu-Juan Shen
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Chinese Center for Tropical Diseases Research; WHO Collaborating Centre for Tropical Diseases; National Center for International Research on Tropical Diseases, Ministry of Science and Technology; Key Laboratory of Parasite and Vector Biology, National Health Commission, Shanghai 200025, China.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2019 May 23;31(2):165-168. doi: 10.16250/j.32.1374.2018233.
To compare the performance of modified Kato-Katz thick smear method (KK method) and PCR assay in field detection of in human fecal samples, which provides insight into the selection of tools for detecting .
Based on the epidemiological investigation of human infections in Tengxian County of Guangxi Zhuang Autonomous Region in 2016, a total of 133 fecal samples were randomly selected and stored at -20 ℃. All fecal samples were detected for infection using KK method and PCR assay, and the detection rate was compared between the two techniques. In addition, test was used to evaluate the consistency between the two methods.
Among all fecal samples, the overall detection rate of was 77.44% (103/133), and the detection rate was significantly higher by PCR assay (70.68%, 93/133) than by KK method (57.14%, 76/133) ( = 26.15, < 0.01). There were 88.16% (67/76) of the microscopy-positive fecal samples positive for PCR assay, and 47.37% (27/57) of the microscopy-negative fecal samples positive for PCR assay. The detection rate of by PCR assay (94.74%, 18/19) was higher in fecal samples with EPG of > 1 000 than in samples with EPG of < 1 000 (85.96%, 49/57) ( = 1.05, = 0.436). The consistency of the detection rate of was moderate between the KK method and PCR assay ( = 0.73).
The detection rate of by PCR assay is significantly higher than by KK method. In low-endemic areas of infections, the combination of KK method and PCR assay is suggested, so as to improve the detection rate.
比较改良加藤厚涂片法（KK法）与聚合酶链反应（PCR）检测人体粪便样本中[病原体名称未给出]的效果，为检测[病原体名称未给出]工具的选择提供依据。
基于2016年广西壮族自治区藤县人体[病原体名称未给出]感染的流行病学调查，随机选取133份粪便样本，于-20℃保存。采用KK法和PCR法对所有粪便样本进行[病原体名称未给出]感染检测，并比较两种技术的检出率。此外，采用[一致性检验方法未给出]检验评估两种方法的一致性。
所有粪便样本中，[病原体名称未给出]的总体检出率为77.44%（103/133），PCR法的检出率（70.68%，93/133）显著高于KK法（57.14%，76/133）（χ² = 26.15，P < 0.01）。镜检阳性粪便样本中88.16%（67/76）PCR检测呈阳性，镜检阴性粪便样本中47.37%（27/57）PCR检测呈阳性。虫卵计数（EPG）>1 000的粪便样本中[病原体名称未给出]的PCR检出率（94.74%，18/19）高于EPG<1 000的样本（85.96%，49/57）（χ² = 1.05，P = 0.436）。KK法与PCR法检测[病原体名称未给出]的检出率一致性为中等（κ = 0.73）。
PCR法检测[病原体名称未给出]的检出率显著高于KK法。在[病原体名称未给出]感染低流行地区，建议联合使用KK法和PCR法，以提高检出率。
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