Greco A, Vaipan D V, Tkachuk V A, Penkov D N
The FIRC Institute of Molecular Oncology, Milan, 20139 Italy.
Moscow State University, Moscow, 119234 Russia.
Mol Biol (Mosk). 2019 May-Jun;53(3):497-501. doi: 10.1134/S0026898419030078.
Homeodomain transcription factors play a significant role in adipocyte differentiation. The role of Pbx1 and Prep1, proteins of the TALE family (the three amino acid loop extension), was previously established in adipocyte differentiation of mesenchymal stromal cells and 3T3-L1 cell line. In this study, with the use of RNA interference technology we show that another transcription factor from the same family, Meis1, which is a core protein of mature cardiomyocytes, represses adipogenesis to a greater degree than its paralog Meis2. A number of Meis target genes, markers of adipocytes, are identified. This may indicate the transcriptional mechanism of the effect of Meis1 on the adipocyte differentiation of mouse preadipocytes.
同源结构域转录因子在脂肪细胞分化中起重要作用。TALE家族（三氨基酸环延伸）的蛋白质Pbx1和Prep1的作用先前已在间充质基质细胞和3T3-L1细胞系的脂肪细胞分化中得到证实。在本研究中，我们利用RNA干扰技术表明，来自同一家族的另一种转录因子Meis1（成熟心肌细胞的核心蛋白）比其旁系同源物Meis2更能抑制脂肪生成。鉴定出了一些Meis靶基因，即脂肪细胞的标志物。这可能表明Meis1对小鼠前脂肪细胞脂肪细胞分化作用的转录机制。
