[耳穴电刺激联合声掩蔽对耳鸣大鼠听觉皮层中CREB、BDNF和TrkB表达的影响]

[Effect of electrical stimulation at auricular points combined with sound masking on the expression of CREB, BDNF and TrkB in the auditory cortex of tinnitus rats].

作者信息

Yang Song-Bai, Peng Li-Yan, Mei Zhi-Gang, Tan Ling-Jing, Huang Ya-Guang, Feng Zhi-Tao, Cai San-Jin, Ma Wen-Han, Zhang Ding-Qi

机构信息

Yichang Hospital of TCM, College of TCM, China Three Gorges University, Yichang 443003, Hubei Province, China.

Department of Otorhinolaryngology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology.

出版信息

Zhongguo Zhen Jiu. 2019 Jun 12;39(6):637-42. doi: 10.13703/j.0255-2930.2019.06.018.

DOI:10.13703/j.0255-2930.2019.06.018

PMID:31190502

Abstract

OBJECTIVE

To explore the effect of electrical stimulation at auricular points (EAS) combined with sound masking on the expression of cAMP-response element binding protein (CREB), brain-derived neurotrophic factor (BDNF) and tyrosine receptor kinase B (TrkB) in the auditory cortex of tinnitus rats.

METHODS

A total of 27 adult male SD rats were randomly divided into a control group, a model group and an EAS group. The rats in the model group and the EAS group were intervened with intraperitoneal injection of sodium salicylate to induce tinnitus model, while the rats in the control group were intervened with injection of 0.9% NaCl solution. After the model was successfully established, the rats in the EAS group were treated with electrical stimulation at "Shenmen" (TF) and "Yidan" (CO), combined with sound masking; the treatment was given once a day for 15 days. The gap prepulse inhibition of acoustic startle (GPIAS) and prepulse inhibition (PPI) testing were performed using the acoustic startle reflex starter package for rats. The expression of BDNF, TrkB, CREB and p-CREB in the auditory cortex of each group were measured with Western Blot analysis.

RESULTS

① Compared with the control group, the GPIAS values in 12 kHz, 16 kHz, 20 kHz and 28 kHz were significantly decreased in the model group (all <0.05); compared with the model group, GPIAS values in 12 kHz, 16 kHz, 20 kHz and 28 kHz were significantly increased in the EAS group (all <0.05). ② Compared with the control group, the expression of BDNF and p-CREB in the model group was significantly increased (<0.01), and the expression of TrkB in the model group was significantly increased (<0.05); the differences of expression of BDNF, TrkB, CREB and p-CREB between the model group and the EAS group had no statistics significance (all >0.05).

CONCLUSION

EAS could improve the GPIAS values of high-frequency background sound in tinnitus rats, which may be related with the upregulation of the BDNF/TrkB/CREB signaling pathway in the auditory cortex, leading to the reversion of the maladaptive plasticity.

摘要

目的

探讨耳穴电刺激（EAS）联合声掩蔽对耳鸣大鼠听觉皮层中环磷酸腺苷反应元件结合蛋白（CREB）、脑源性神经营养因子（BDNF）和酪氨酸受体激酶B（TrkB）表达的影响。

方法

将27只成年雄性SD大鼠随机分为对照组、模型组和耳穴电刺激组。模型组和耳穴电刺激组大鼠腹腔注射水杨酸钠诱导耳鸣模型，对照组大鼠注射0.9%氯化钠溶液。模型成功建立后，耳穴电刺激组大鼠给予“神门”（TF）和“胰胆”（CO）耳穴电刺激，联合声掩蔽；每天治疗1次，共15天。使用大鼠听觉惊吓反射启动包进行声惊吓的间隔前脉冲抑制（GPIAS）和前脉冲抑制（PPI）测试。采用蛋白质免疫印迹分析检测各组大鼠听觉皮层中BDNF、TrkB、CREB和磷酸化CREB（p-CREB）的表达。

结果

①与对照组相比，模型组12kHz、16kHz、20kHz和28kHz的GPIAS值显著降低（均<0.05）；与模型组相比，耳穴电刺激组12kHz、16kHz、20kHz和28kHz的GPIAS值显著升高（均<0.05）。②与对照组相比，模型组BDNF和p-CREB的表达显著升高（<0.01），模型组TrkB的表达显著升高（<0.05）；模型组与耳穴电刺激组BDNF、TrkB、CREB和p-CREB表达的差异无统计学意义（均>0.05）。