Song Rui-Biao, Lou Wei-Hua
Clin Lab. 2015;61(9):1113-8. doi: 10.7754/clin.lab.2015.150212.
This study investigated the effects of monosialotetrahexosylganglioside (GM1) on the expression of N-methyl-D-aspartate receptor subunit 2B (NR2B) and phosphorylated (p)-cyclic AMP response element-binding protein (CREB) in the auditory cortex of rats with tinnitus.
Tinnitus-like behavior in rats was tested with the gap prepulse inhibition of acoustic startle paradigm. We then investigated the NR2B mRNA and protein and p-CREB protein levels in the auditory cortex of tinnitus rats compared with normal rats.
Rats treated for 4 days with salicylate exhibited tinnitus. NR2B mRNA and protein and p-CREB protein levels were upregulated in these animals, with expression returning to normal levels 14 days after cessation of treatment; baseline levels of NR2B and p-CREB were also restored by GM1 administration.
These data suggest that chronic salicylate administration induces tinnitus via upregulation of p-CREB and NR2B expression, and that GM1 can potentially be used to treat tinnitus.
本研究调查了单唾液酸四己糖神经节苷脂（GM1）对耳鸣大鼠听觉皮层中N-甲基-D-天冬氨酸受体亚基2B（NR2B）及磷酸化（p）-环磷酸腺苷反应元件结合蛋白（CREB）表达的影响。
采用听觉惊吓反应前脉冲抑制模型检测大鼠的耳鸣样行为。然后，我们研究了耳鸣大鼠与正常大鼠相比，听觉皮层中NR2B mRNA、蛋白及p-CREB蛋白水平。
用阿司匹林治疗4天的大鼠出现耳鸣。这些动物的NR2B mRNA、蛋白及p-CREB蛋白水平上调，治疗停止14天后表达恢复正常水平；给予GM1也可使NR2B和p-CREB的基线水平恢复。
这些数据表明，长期给予阿司匹林通过上调p-CREB和NR2B的表达诱导耳鸣，GM1可能可用于治疗耳鸣。
