Two novel glycosides, apostichoposide A () and B (), were isolated from the viscera of Chinese sea cucumbers (, Selenka) collected in the Bohai sea. Both the isolated glycosides were characterized by non-holostane type aglycones having 18(16)-lactone and 7(8)-double bond. Cytotoxic activities of the two compounds were evaluated against three human cancer cell lines. Compound had adequate cytotoxic activity against MGC-803 and PC-3M cell lines. Our results indicated that glycosides present in viscera are an important high value resource for biotechnological applications.