Department of Experimental Oncology, Institute for Oncology and Radiology of Serbia, Pasterova, Belgrade, Serbia.
Department of Medical Oncology, Institute for Oncology and Radiology of Serbia, Pasterova, Belgrade, Serbia.
Int J Biol Markers. 2019 Sep;34(3):262-268. doi: 10.1177/1724600819850178. Epub 2019 Jun 13.
Aberrant expression of different tight junction proteins, including the junctional adhesion molecule-A (JAM-A), has been frequently reported in association with tumor progression of several malignancies. To our knowledge, this is the first study examining the clinical significance of gene expression in epithelial ovarian cancer.
expression levels in 44 epithelial ovarian cancer and 12 benign formalin-fixed paraffin-embedded samples were determined by reverse transcription quantitative polymerase chain reaction. Receiver operating characteristic (ROC) curve analysis was used to determine the diagnostic and prognostic potential of . Associations between expression and clinicopathological characteristics of epithelial ovarian cancer were analyzed using Fisher's exact test. The Kaplan-Meier method and univariate Cox regression analysis were used for the survival analysis. ⩽ 0.05 was considered statistically significant.
ROC curve analyses showed that gene expression exhibits both diagnostic and prognostic performance in epithelial ovarian cancer (area under the curve (AUC) 0.640, 95% confidence interval (CI) 0.488, 0.792, sensitivity 43.18%, specificity 100% and AUC 0.621, 95% CI 0.427, 0.816, sensitivity 52.63%, specificity 85%, respectively). expression was significantly associated with International Federation of Gynecologists and Obstetricians (FIGO) stage ( =0.049) and the Kaplan-Meier method demonstrated that patients with high expression of had significantly worse overall survival compared to patients with low expression ( =0.004). Moreover, univariate Cox regression analysis showed that FIGO stage, peritoneal metastasis, residual tumor and expression were significantly associated with reduced overall survival in epithelial ovarian cancer.
Our results indicate that high levels of expression are associated with an advanced clinicopathological feature and may have diagnostic potential; also, it could be a predictor of poor overall survival in patients with epithelial ovarian cancer.
已有研究表明，多种恶性肿瘤的进展与不同紧密连接蛋白（包括连接黏附分子 A[JAM-A]）的异常表达有关。据我们所知，这是首次研究基因在卵巢上皮性癌中的表达的临床意义。
采用反转录定量聚合酶链反应检测 44 例卵巢上皮性癌和 12 例良性福尔马林固定石蜡包埋组织中基因的表达水平。采用受试者工作特征（ROC）曲线分析确定基因表达对上皮性卵巢癌的诊断和预后价值。采用 Fisher 确切概率法分析基因表达与上皮性卵巢癌临床病理特征的相关性。采用 Kaplan-Meier 法和单因素 Cox 回归分析进行生存分析。以 P ⩽ 0.05 为差异有统计学意义。
ROC 曲线分析显示，基因表达在上皮性卵巢癌中具有诊断和预后性能（曲线下面积（AUC）0.640，95%置信区间（CI）0.488～0.792，敏感度 43.18%，特异度 100%和 AUC 0.621，95%CI 0.427～0.816，敏感度 52.63%，特异度 85%）。基因表达与国际妇产科联盟（FIGO）分期显著相关（P=0.049）。Kaplan-Meier 法表明，基因高表达患者的总生存时间明显短于低表达患者（P=0.004）。此外，单因素 Cox 回归分析显示，FIGO 分期、腹膜转移、残留肿瘤和基因表达与上皮性卵巢癌患者总生存时间缩短显著相关。
本研究结果表明，基因高表达与较晚期的临床病理特征相关，可能具有诊断潜力，并且可能是上皮性卵巢癌患者总生存时间的不良预后因素。
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