Gupta Renu, Chauhan Sneh Lata, Kumar Sunil, Jindal Naresh, Mahajan N K, Joshi V G
Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India.
Department of Animal Biotechnology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India.
Vet World. 2019;12(4):609-613. doi: 10.14202/vetworld.2019.609-613. Epub 2019 Apr 25.
The present study was conducted with the following aims: (i) To screen the spp. isolates recovered from suspected cases of fowl typhoid for carriage of Class 1 integrons and analyze their association with antimicrobial resistance and (ii) to carry out molecular characterization and phylogenetic analysis of Class 1 integron-integrase () gene.
A total of 43 isolates were subjected to polymerase chain reaction (PCR) assay to determine the presence of Class1 . Differences between different serotypes in relation to their carriage of integrons and the differences between strains containing or not containing an integron and being resistant to different antimicrobials were analyzed by Fisher exact test using STATA™ (StataCorp, College Station, TX). Phylogenetic analysis was carried out using MEGA6 software.
Out of 43 isolates, 40 (93.02%) were found positive for Class 1 integrons. 35/40 (87.5%) -positive isolates were multidrug resistance (MDR) (resistant to ≥4 antibiotics), which support the hypothesis of an association between the presence of Class 1 integrons and emerging MDR in . There was no significant difference among isolates resistant to different antimicrobials in Class 1 integron carrying isolates and the Class 1 integron negative isolates (p<0.05). Further, there was no significant difference among different serotypes in respect of their carriage of Class 1 integrons.
It can be concluded that the high prevalence of Class 1 integrons indicates a high potential of isolates for horizontal transmission of antimicrobial genes, especially among Gram-negative organisms.
本研究旨在实现以下目标：（i）筛选从疑似禽伤寒病例中分离出的 菌属菌株，以检测 1 类整合子的携带情况，并分析其与抗菌药物耐药性的关联；（ii）对 1 类整合子整合酶（）基因进行分子特征分析和系统发育分析。
共对 43 株 菌属菌株进行聚合酶链反应（PCR）检测，以确定 1 类 的存在情况。使用 STATA™（StataCorp，美国德克萨斯州大学城）软件通过 Fisher 精确检验分析不同血清型在整合子携带方面的差异，以及含有或不含有整合子且对不同抗菌药物耐药的菌株之间的差异。使用 MEGA6 软件进行系统发育分析。
在 43 株分离菌株中，40 株（93.02%）被检测出 1 类整合子呈阳性。40 株整合子阳性分离株中有 35 株（87.5%）为多重耐药（MDR）（对≥4 种抗生素耐药），这支持了 1 类整合子的存在与 菌属中新兴多重耐药之间存在关联的假设。携带 1 类整合子的分离株和 1 类整合子阴性分离株中，对不同抗菌药物耐药的分离株之间无显著差异（p<0.05）。此外，不同血清型在 1 类整合子携带方面也无显著差异。
可以得出结论，1 类整合子的高流行率表明 菌属分离株具有较高水平传播抗菌基因的潜力，尤其是在革兰氏阴性菌中。
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