Clinical Laboratory of Weifang People's Hospital, 151 Guangwen Street, Weifang, Shandong Province, PR China.
Department of Clinical Laboratory, Affiliated Hospital of Qingdao Binhai University, Qingdao, Shandong Province, PR China.
J Med Microbiol. 2022 Sep;71(9). doi: 10.1099/jmm.0.001574.
The emergence of antibiotic-resistant isolates is a global concern and has been attributed to the indiscriminate use of antibiotics in humans and animals. Integrons are mobile gene elements closely related to bacterial drug resistance. Among them, class 1 integrons containing various resistance gene cassettes could play an important role in disseminating and maintaining antibiotic resistance in isolates.. class 1 integrons have a relationship with drug resistance.. This study aims to investigate the distribution of class 1 integrons and their variable regional molecular characteristics, as well as the diversity of the promoters and drug sensitivity among strains.. A total of 111 strains, collected between 2018 and 2020, underwent fully automated bacterial identification using the VITEK 2 Compact system and an antibiotic sensitivity test. PCR was employed to screen class 1 integrase genes () and integron variable regions, while promoter type and variable region gene cassette characteristics were determined using sequencing analysis.. A total of 24 -positive strains were detected in 111 strains. Moreover, -positive strains exhibited statistically significant resistance to ceftazidime, ciprofloxacin, levofloxacin ceftriaxone, trimethoprim/sulfamethoxazole and azithromycin compared to integron-negative strains (<0.05). The multidrug resistance rate of -positive strains was significantly higher than that of negative strains. Variable regions were observed in 6 of the 24 -positive strains. Four gene cassettes were detected, namely , , and . Finally, 3 types of class 1 integron variable region promoters were identified in 24 strains, including PcW, PcH1 and PcW; they are all relatively weak promoters.. The integron and the drug resistance genes carried by integron have a certain relationship with drug resistance.
抗生素耐药 分离株的出现是一个全球性的关注问题，其归因于抗生素在人类和动物中的滥用。整合子是与细菌耐药性密切相关的可移动基因元件。其中，含有各种耐药基因盒的 1 类整合子在 分离株中抗生素耐药性的传播和维持中可能发挥重要作用。1 类整合子与 耐药性有关。本研究旨在调查 1 类整合子的分布及其可变区分子特征，以及 株中启动子和药物敏感性的多样性。共收集了 111 株于 2018 年至 2020 年期间采集的菌株，采用 VITEK 2 Compact 系统进行全自动细菌鉴定和药敏试验。PCR 法筛选 1 类整合酶基因（）和整合子可变区，测序分析确定启动子类型和可变区基因盒特征。在 111 株菌株中检测到 24 株 -阳性菌株。此外，与整合子阴性菌株相比，-阳性菌株对头孢他啶、环丙沙星、左氧氟沙星、头孢曲松、复方新诺明和阿奇霉素的耐药率具有统计学意义（<0.05）。-阳性菌株的多重耐药率明显高于阴性菌株。在 24 株 -阳性菌株中观察到 6 株存在可变区。检测到 4 个基因盒，分别为 、 、 和 。最后，在 24 株菌中鉴定出 3 种 1 类整合子可变区启动子类型，包括 PcW、PcH1 和 PcW；它们都是相对较弱的启动子。整合子及其携带的耐药基因与耐药性有一定关系。
