School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.
Lab Chip. 2019 Jul 9;19(14):2435-2443. doi: 10.1039/c9lc00163h.
We report a microfluidic fluorescence activated cell-sorting (μFACS) device that employs traveling surface acoustic waves (TSAW) to sort cells at rates comparable to conventional jet-in-air FACS machines, with high purity and viability. The device combines inertial flow focusing and sheath flow to align and evenly space cells, improving the sorting accuracy and screening rate. We sort with an interdigital transducer (IDT) whose tapered geometry allows precise positioning of the TSAW for optimal cell sorting. We sort three different cell lines at several kHz, at cell velocities exceeding one meter per second, while maintaining both sorting purity and cell viability at around 90% simultaneously.
我们报告了一种微流控荧光激活细胞分选（μFACS）装置，该装置采用行波（TSAW）对细胞进行分选，其分选速度可与传统的喷气式 FACS 仪器相媲美，同时具有较高的纯度和活力。该装置结合了惯性流聚焦和鞘流来对齐和均匀间隔细胞，从而提高了分选精度和筛选率。我们使用叉指换能器（IDT）进行分选，其锥形几何形状允许精确地定位 TSAW 以实现最佳的细胞分选。我们以几 kHz 的频率对三种不同的细胞系进行分选，细胞速度超过每秒一米，同时同时保持分选纯度和细胞活力在 90%左右。
Zotero 插件