通过16S-23S内转录间隔区PCR对产细胞毒素分离株进行分子分型。
Molecular typing of cytotoxin-producing isolates by 16S-23S internal transcribed spacer PCR.
作者信息
Soltan Dallal M M, Validi M, Douraghi M, Bakhshi B
机构信息
Department of Food Microbiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Food Microbiology Research Centre, Tehran University of Medical Sciences, Tehran, Iran.
出版信息
New Microbes New Infect. 2019 Apr 9;30:100545. doi: 10.1016/j.nmni.2019.100545. eCollection 2019 Jul.
Cytotoxin is one of the important pathogenic factors, which plays a role in the virulence of . The aim of this study was to investigate molecular typing of clinical isolates of the cytotoxin-producing using internal transcribed spacer (ITS) PCR. A total of 75 isolates of were isolated from clinical samples; they were verified as by standard microbiological tests and PCR. Production of toxin determines the cytotoxic effects on HEp-2 cells. The genetic diversity of isolates of the cytotoxin-producing were defined by ITS-PCR. Of all the isolates investigated, five strains isolated from stool cultures, two strains from blood samples, one strain from a wound and one strain isolated from urine had cytotoxic effects on HEp-2 cells. The ITS-PCR patterns showed genetic diversity among cytotoxin-producing isolates. The ITS-PCR method had good discriminatory power; performance of this method and interpretation of the results were easy and repeatable. Five genetic diversity patterns were identified by ITS-PCR.
细胞毒素是重要的致病因素之一，在……的毒力中起作用。本研究的目的是使用内转录间隔区（ITS）PCR研究产细胞毒素……临床分离株的分子分型。总共从临床样本中分离出75株……；通过标准微生物学检测和PCR将它们鉴定为……。毒素的产生决定了对HEp-2细胞的细胞毒性作用。产细胞毒素……分离株的遗传多样性通过ITS-PCR确定。在所有研究的分离株中，从粪便培养物中分离出的5株……菌株、从血液样本中分离出的2株、从伤口分离出的1株和从尿液中分离出的1株对HEp-2细胞具有细胞毒性作用。ITS-PCR图谱显示产细胞毒素分离株之间存在遗传多样性。ITS-PCR方法具有良好的鉴别能力；该方法的操作和结果解释简单且可重复。通过ITS-PCR鉴定出五种遗传多样性模式。
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