Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Department of Food Microbiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Microb Pathog. 2017 Dec;113:1-4. doi: 10.1016/j.micpath.2017.09.063. Epub 2017 Sep 29.
Klebsiella oxytoca is an opportunistic pathogen which damages intestinal epithelium through producing cytotoxin tilivalline. This toxin plays a role in the pathogenesis of bacteria and is the main virulence factor which leads to antibiotic-associated hemorrhagic colitis progress.
In this study, we collected a total of 75 K. oxytoca strains isolated from the stool, urine, blood, wounds, and sputum and evaluated them in terms of the production of toxins; we detected their cytotoxic effects on HEp-2 cells.
Of all the isolates, five K. oxytoca strains isolated from the stool cultures, two strains isolated from the blood cultures, one strains isolated from the wound cultures, and one strains isolated from the urine cultures had cytotoxic effects on HEp-2 cells. The strains isolated from sputum cultures had no cytotoxic effects on HEp-2 cells.
In the current study, the majority of strains isolated from the stool of the patients included cytopathic effects on HEp-2 cells.
产酸克雷伯菌是一种机会致病菌，通过产生细胞毒素 tilivalline 来破坏肠道上皮细胞。这种毒素在细菌的发病机制中起作用，是导致抗生素相关性出血性结肠炎进展的主要毒力因子。
本研究共收集了 75 株来自粪便、尿液、血液、伤口和痰液的产酸克雷伯菌菌株，并对其毒素产生情况进行评估；检测了它们对 HEp-2 细胞的细胞毒性作用。
在所分离的菌株中，有 5 株来自粪便培养物、2 株来自血液培养物、1 株来自伤口培养物和 1 株来自尿液培养物的产酸克雷伯菌菌株对 HEp-2 细胞具有细胞毒性作用。来自痰液培养物的菌株对 HEp-2 细胞没有细胞毒性作用。
在本研究中，大多数从患者粪便中分离的菌株对 HEp-2 细胞具有细胞病变效应。
Zotero 插件