Sorimachi K, Yasumura Y
Department of Microbiology, Dokkyo University School of Medicine, Tochigi, Japan.
Endocrinol Jpn. 1987 Aug;34(4):615-20. doi: 10.1507/endocrj1954.34.615.
When monkey hepatocarcinoma cells (NCLP-6E) were treated with 10% of various serum preparations for 24 h at 37 degrees C, nonphenolic ring deiodinase activity increased 2.0- to 2.3-fold. Phenolic ring deiodinase activity also increased 0.9- to 2.1-fold. Dialysis of the sera enhanced the effect on deiodinase activities in some preparations, but reduced activity in other serum preparations. Similarly, a 1.3- to 3.1-fold increase in phenolic ring deiodinase activity was observed in rat hepatoma cells (R-Y121B). In this case, dialysis usually reduced the effect of the sera. It is concluded that both large molecule(s) (undialyzable) and small molecule(s) (dialyzable) in serum contribute to the regulation of phenolic and nonphenolic ring deiodinase activity in NCLP-6E and R-Y121B cells.
当猴肝癌细胞（NCLP - 6E）在37℃下用10%的各种血清制剂处理24小时时，非酚环脱碘酶活性增加了2.0至2.3倍。酚环脱碘酶活性也增加了0.9至2.1倍。血清的透析在某些制剂中增强了对脱碘酶活性的影响，但在其他血清制剂中降低了活性。同样，在大鼠肝癌细胞（R - Y121B）中观察到酚环脱碘酶活性增加了1.3至3.1倍。在这种情况下，透析通常会降低血清的作用。结论是血清中的大分子（不可透析）和小分子（可透析）都有助于调节NCLP - 6E和R - Y121B细胞中酚环和非酚环脱碘酶的活性。
