秋水仙碱可激活生长停滞的大鼠3Y1细胞中细胞周期依赖性基因。

Colchicine activates cell cycle-dependent genes in growth-arrested rat 3Y1 cells.

作者信息

Miura M, Ninomiya-Tsuji J, Tsuji Y, Ishibashi S, Ide T

机构信息

Department of Physiological Chemistry, Hiroshima University School of Medicine, Japan.

出版信息

Exp Cell Res. 1987 Nov;173(1):294-8. doi: 10.1016/0014-4827(87)90356-9.

DOI:10.1016/0014-4827(87)90356-9

PMID:3119359

Abstract

When growth-arrested 3Y1 cells (Fischer rat fibroblasts) were exposed to 3 X 10(-5) M colchicine, they entered S phase after a 12-h lag period which is the same as that in serum-stimulated cells. The expression of genes such as c-fos, c-myc, JE, KC, ornithine decarboxylase, and histone H3, analyzed by Northern blotting, increased in a cell-cycle dependent manner after colchicine treatment. The increased level of mRNAs was much smaller in colchicine-stimulated cells than in serum-stimulated cells, corresponding to the lower frequency of the former cells entering S phase. The course of the prereplicative phase seems to be similar in terms of the expression of cell cycle-dependent genes in cells stimulated with colchicine and in those stimulated with serum.

摘要

当生长停滞的3Y1细胞（Fischer大鼠成纤维细胞）暴露于3×10⁻⁵ M秋水仙碱时，它们在12小时的延迟期后进入S期，这与血清刺激的细胞相同。通过Northern印迹分析，c-fos、c-myc、JE、KC、鸟氨酸脱羧酶和组蛋白H3等基因的表达在秋水仙碱处理后以细胞周期依赖性方式增加。秋水仙碱刺激的细胞中mRNA增加的水平远低于血清刺激的细胞，这与前者细胞进入S期的频率较低相对应。就秋水仙碱刺激的细胞和血清刺激的细胞中细胞周期依赖性基因的表达而言，复制前期的过程似乎相似。