Takasuka T, Ishibashi S, Ide T
Department of Physiological Chemistry, Hiroshima University School of Medicine, Japan.
Exp Cell Res. 1987 Nov;173(1):287-93. doi: 10.1016/0014-4827(87)90355-7.
GC-7 cells, a cell line from African green monkey kidney, which had been growth arrested in G0 phase by serum deprivation, entered S phase 15 h after serum stimulation. They were blocked from entering S phase in the presence of 0.6 micrograms/ml of cytochalasin D. The cells growth arrested between G0 and S phase by cytochalasin D entered S phase 6 h following the removal of the drug. The progression of S, G2, and M phases was not affected by cytochalasin D. On the other hand, when G0-arrested GC-7 cells were stimulated with serum for 23 h up to a late S/G2 phase and then cultured in the presence of cytochalasin D, or when an exponentially growing culture was treated with the drug, the cells were growth arrested at a point 15 h, not 6 h, before the next S phase. This point of growth arrest is kinetically similar to G0 phase, both occur 15 h before S phase, but is different from G0 in terms of c-fos expression after release from the block.
GC-7细胞系源自非洲绿猴肾，通过血清剥夺使其生长停滞于G0期，血清刺激15小时后进入S期。在存在0.6微克/毫升细胞松弛素D的情况下，它们被阻止进入S期。被细胞松弛素D阻滞在G0和S期之间的细胞在去除药物后6小时进入S期。S期、G2期和M期的进程不受细胞松弛素D的影响。另一方面，当处于G0期停滞的GC-7细胞用血清刺激23小时直至晚期S/G2期，然后在细胞松弛素D存在的情况下培养，或者当对数生长期的培养物用该药物处理时，细胞在距离下一个S期15小时而非6小时的时间点生长停滞。这个生长停滞点在动力学上与G0期相似，两者均在S期前15小时出现，但在从阻滞中释放后c-fos表达方面与G0期不同。
