Department of Biomedical Materials Engineering, Kangwon National University, Chuncheon, 24341, Republic of Korea.
GtreeBNT Co., Ltd., Seongnam-Si, 13554, Republic of Korea.
J Nanosci Nanotechnol. 2019 Dec 1;19(12):7920-7925. doi: 10.1166/jnn.2019.16860.
Various biocompatible polymers have been developed using electrospun nanofibers for local drug delivery matrices, but many of them are non-FDA-approved polymers or chemicals. Material safety should be considered in biomedical devices, but the effectiveness of electrospun nanofibers is limited with only the approved chemicals. Therefore, we considered the material in FDA-approved polymers and solvents and developed nanofibers using the general additives in the pharmaceutical industry, such as hypromellose, poly(vinyl alcohol) (PVA), and Gellan. The nanofiber, mainly composed of hypromellose and PVA, was applied to a local peptide drug delivery system. Electrostatically complexed Gellan and peptide were loaded in the nanofiber by co-electrospinning. The morphology of the nanofibers with different PVA blending ratios was visualized by scanning electron microscope. The nanofiber composed of only hydrophilic polymers quickly wetted in water and became a transparent gel-like lump. A drug release test of peptide-loaded nanofiber was performed resulted in 37% of initial burst release suppression with the gellan and peptide loaded nanofiber compared with solely peptide loaded hypromellose nanofiber. In addition, higher PVA blending to hypromellose was slightly effective for sustained release of peptide compared with pure peptide-loaded hypromellose nanofiber. Therefore, we suggest the potential application of hypromellose/PVA nanofiber-loaded Gellan/peptide complex to a mucosal layer drug delivery patch.
已经开发出了各种使用电纺纳米纤维的生物相容性聚合物，用于局部药物输送基质，但其中许多是未经 FDA 批准的聚合物或化学品。生物医学设备应考虑材料安全性，但由于仅使用批准的化学品，电纺纳米纤维的效果有限。因此，我们考虑了 FDA 批准的聚合物和溶剂中的材料，并使用制药行业中的常规添加剂（如羟丙甲纤维素、聚乙烯醇（PVA）和结冷胶）开发了纳米纤维。主要由羟丙甲纤维素和 PVA 组成的纳米纤维应用于局部肽药物输送系统。通过共电纺将静电复合的结冷胶和肽装载到纳米纤维中。通过扫描电子显微镜观察不同 PVA 混合比的纳米纤维的形态。由仅亲水性聚合物组成的纳米纤维在水中迅速润湿，并变成透明的凝胶状块状物。载肽纳米纤维的药物释放试验表明，与仅载肽的羟丙甲纤维素纳米纤维相比，载有结冷胶和肽的纳米纤维的初始突释抑制了 37%。此外，与纯载肽的羟丙甲纤维素纳米纤维相比，较高比例的 PVA 混合到羟丙甲纤维素中对肽的持续释放略有效果。因此，我们建议将载有结冷胶/肽复合物的羟丙甲纤维素/PVA 纳米纤维潜在应用于粘膜层药物输送贴剂。
