利用茄科异源嫁接系统鉴定韧皮部移动mRNA

Identification of Phloem Mobile mRNAs Using the Solanaceae Heterograft System.

作者信息

Xia Chao, Zheng Yi, Huang Jing, Fei Zhangjun, Zhang Cankui

机构信息

Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China.

Department of Agronomy and Purdue Center for Plant Biology, Purdue University, West Lafayette, IN, USA.

出版信息

Methods Mol Biol. 2019;2014:421-431. doi: 10.1007/978-1-4939-9562-2_32.

DOI:10.1007/978-1-4939-9562-2_32

PMID:31197813

Abstract

Large numbers of mRNAs move in the phloem and some may function as signals to exert important physiological functions in the distal recipient organs. Generating an authentic list of phloem mobile mRNA is a prerequisite for elucidating their physiological functions. Nicotiana benthamiana can be used as a scion to graft on a tomato (Solanum lycopersicum) rootstock. Thereby, shoot-to-root mobile N. benthamiana mRNAs transported via the phloem can be identified from the root of the tomato rootstock. Due to the close relationship and similar genome sequences of the two species, stringent informatics procedures should be applied to avoid false identification. This heterograft system can be used to study physiological processes associated with mRNAs that are mobile under either normal or adverse growth condition.

摘要

大量的信使核糖核酸（mRNA）在韧皮部中移动，其中一些可能作为信号在远端受体器官中发挥重要的生理功能。生成一份真实的韧皮部可移动mRNA列表是阐明其生理功能的先决条件。本氏烟草可以用作接穗嫁接到番茄（Solanum lycopersicum）砧木上。由此，通过韧皮部运输的从地上部到根部可移动的本氏烟草mRNA可以从番茄砧木的根部鉴定出来。由于这两个物种关系密切且基因组序列相似，应采用严格的信息学程序以避免错误鉴定。这种异种嫁接系统可用于研究与在正常或逆境生长条件下可移动的mRNA相关的生理过程。