以蚜虫作为生物电极测量完整筛管分子中的电位波

Measurement of Electropotential Waves in Intact Sieve Elements Using Aphids as Bioelectrodes.

作者信息

Furch Alexandra C U, Zimmermann Matthias R, Will Torsten

机构信息

Matthias Schleiden Institute of Genetics, Bioinformatics and Molecular Botany, Faculty of Biological Science, Friedrich-Schiller-University Jena, Jena, Germany.

Julius Kuehn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Quedlinburg, Germany.

出版信息

Methods Mol Biol. 2019;2014:449-457. doi: 10.1007/978-1-4939-9562-2_35.

DOI:10.1007/978-1-4939-9562-2_35

PMID:31197816

Abstract

Electropotential waves (EPWs) are thought to transmit sudden and profound physiological changes between plant organs. The recording of EPWs can be performed via extracellular or intracellular probes. Both approaches have advantages and disadvantages. Since the phloem is responsible for long distance transport of the most forms of EPWs, the direct measurement in sieve elements is preferable. The conductance using glass microelectrodes inserted into free lying sieve elements is described in Chapter 34 . In this chapter the measurement of EPWs by using aphids as bioelectrodes is described in detail.The electrical penetration graph technique (EPG) takes advantage of the flexible mouthparts (stylet) of aphids, which specifically penetrate into sieve elements. The use of aphids as bioelectrodes enables multiple electrode recordings and long-distance observations of EPWs. Importantly, this method allows for noninvasive, intracellular measurements.

摘要

电势波（EPWs）被认为在植物器官之间传递突然而深刻的生理变化。电势波的记录可以通过细胞外或细胞内探针进行。这两种方法都有优缺点。由于韧皮部负责大多数形式的电势波的长距离运输，因此在筛管分子中进行直接测量更为可取。第34章描述了使用插入自由筛管分子的玻璃微电极测量电导率的方法。在本章中，将详细描述使用蚜虫作为生物电极来测量电势波。电穿透图技术（EPG）利用了蚜虫灵活的口器（口针），口针能特异性地穿透到筛管分子中。使用蚜虫作为生物电极能够对电势波进行多电极记录和远距离观察。重要的是，这种方法允许进行非侵入性的细胞内测量。