卫星噬菌体P4在大肠杆菌中的整合。参与位点特异性重组的噬菌体和宿主区域的DNA序列。

Integration of satellite bacteriophage P4 in Escherichia coli. DNA sequences of the phage and host regions involved in site-specific recombination.

作者信息

Pierson L S, Kahn M L

机构信息

Department of Microbiology, Washington State University, Pullman 99164-4340.

出版信息

J Mol Biol. 1987 Aug 5;196(3):487-96. doi: 10.1016/0022-2836(87)90026-x.

DOI:10.1016/0022-2836(87)90026-x

PMID:3119856

Abstract

We determined the DNA sequences of regions essential for bacteriophage P4 integration. A 20 base-pair core sequence in both phage (P4attP) and host (P4attB) attachment regions contains the recombination site. In P4attP this sequence is flanked by five repeated sequences. A 1.3 x 10(3) base open reading frame codes for P4 integrase. Two possible promoters are upstream from P4int. One would be recognized by Escherichia coli RNA polymerase and may be repressed by integrase protein. The second would be recognized by RNA polymerase modified after infection by a P4 helper phage, P2. The P4attB core sequence is the 3' end of a leucine tRNA gene. Downstream from this tRNA in E. coli K-12 is a region homologous to P4int that may be part of a cryptic prophage.

摘要

我们确定了噬菌体P4整合所必需区域的DNA序列。噬菌体（P4attP）和宿主（P4attB）附着区域中的一个20个碱基对的核心序列包含重组位点。在P4attP中，该序列两侧有五个重复序列。一个1.3×10³碱基的开放阅读框编码P4整合酶。P4int上游有两个可能的启动子。一个可被大肠杆菌RNA聚合酶识别，可能被整合酶蛋白抑制。另一个可被感染P4辅助噬菌体P2后修饰的RNA聚合酶识别。P4attB核心序列是亮氨酸tRNA基因的3'端。在大肠杆菌K - 12中，该tRNA下游是一个与P4int同源的区域，可能是一个隐蔽原噬菌体的一部分。