University of Paris-Est, Laboratoire Géomatériaux et Environnement (EA 4508), UPEM, 77454 Marne-la-Vallée, France; University of Limoges, PEIRENE, Equipe Développement d'indicateurs ou prévision de la qualité des eaux, URA IRSTEA, 123 Avenue Albert Thomas, 87060 Limoges Cedex, France.
Department of Thematic Studies-Environmental Change and Biogas Research Center, Linköping University, 581 83 Linköping, Sweden.
Bioresour Technol. 2019 Sep;288:121587. doi: 10.1016/j.biortech.2019.121587. Epub 2019 May 31.
This study evaluates a simultaneous assessment of organic matter (OM) and trace elements (TE) bio-accessibility in substrate and digestate from a full-scale anaerobic digester by a sequential OM extraction method. Simultaneous release of TE was determined along with the extraction of different OM fractions and the effects of extracting reagents on characteristics of OM were evaluated by nuclear magnetic resonance (NMR) spectroscopy. The reagents used for sequential extraction of OM were not enough selective. However, proteins were particularly removed by 0.1 M NaOH, while 72% HSO mainly extracted hemicellulose and cellulose. The OM fractionation allowed for simultaneous extraction of >60% of total As, Cd, Co, Fe, Mn, Ni and Zn, while the extraction was limited for Al, Cr, Cu, Mo, and Pb. In substrate, >50% of total As, Co, Mn and Ni and <40% of total Fe, Zn and Mo were identified in bio-accessible fractions. In digestate, all elements demonstrated poor bio-accessibility except for As.
本研究通过顺序有机质提取方法评估了大规模厌氧消化器中基质和消化物中有机质（OM）和痕量元素（TE）生物可利用性的同步评估。同时确定了 TE 的释放情况，以及不同 OM 级分的提取情况，并通过核磁共振（NMR）光谱评估了提取试剂对 OM 特性的影响。用于 OM 顺序提取的试剂选择性不足。然而，0.1 M NaOH 特别去除了蛋白质，而 72% HSO 主要提取半纤维素和纤维素。OM 分级可同时提取 >60%的总 As、Cd、Co、Fe、Mn、Ni 和 Zn，而 Al、Cr、Cu、Mo 和 Pb 的提取则受到限制。在基质中，>50%的总 As、Co、Mn 和 Ni 和 <40%的总 Fe、Zn 和 Mo 存在于生物可利用级分中。在消化物中，除了 As 外，所有元素的生物可利用性都很差。
