Academy of Military Medical Sciences, Beijing, 100850, China.
Department of Tumor Radiotherapy and Chemotherapy, Yinzhou People's Hospital, Ningbo, 315040, China.
Biochem Biophys Res Commun. 2019 Aug 13;516(1):102-111. doi: 10.1016/j.bbrc.2019.05.182. Epub 2019 Jun 11.
Here, we firstly examined the proliferation and invasion capacities of different osteosarcoma (OS) cell lines, and analyzed the profiling of the differentially expressed circular RNAs(circRNAs). Then, we identified a novel circRNA(circ_101356/circ_0004846, we named circSAMD4 in the present study) and found it was highly expressed in the OS tissues compared to adjacent non-cancerous tissues. Furthermore, we indicated that circSAMD4A promoted OS proliferation in vivo and in vitro. In addition, we proved that circSAMD4A enhances osteosarcoma cells stemness features. In mechanism, circSAMD4A could sponge miR-1244 in OS cells. Moreover, we verified that MDM2 was a target of miR-1244. In conclusion, circSAMD4A/miR-1244/MDM2 regulatory loop might be a promising therapeutic target for OS treatment.
在这里，我们首先检查了不同骨肉瘤（OS）细胞系的增殖和侵袭能力，并分析了差异表达环状 RNA（circRNA）的特征。然后，我们鉴定出一个新型环状 RNA（circ_101356/circ_0004846，在本研究中我们将其命名为 circSAMD4），并发现其在 OS 组织中的表达水平明显高于相邻的非癌组织。此外，我们表明 circSAMD4A 促进了 OS 在体内和体外的增殖。此外，我们证明 circSAMD4A 增强了骨肉瘤细胞的干性特征。在机制上，circSAMD4A 可以在 OS 细胞中吸附 miR-1244。此外，我们验证了 MDM2 是 miR-1244 的靶标。总之，circSAMD4A/miR-1244/MDM2 调节环可能是治疗骨肉瘤的有前途的治疗靶点。
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