辛基酚和壬基酚影响人子宫内膜基质细胞的蜕膜化。
Octylphenol and nonylphenol affect decidualization of human endometrial stromal cells.
机构信息
Laboratory of Stem Cells and Tissue Regeneration, Department of Biotechnology, College of Life Sciences and Biotechnology, Science Campus, Korea University, Seoul, South Korea.
Department of Biomedical Science, CHA University, Seongnam, South Korea.
出版信息
Reprod Toxicol. 2019 Oct;89:13-20. doi: 10.1016/j.reprotox.2019.06.003. Epub 2019 Jun 12.
This study investigated the effect of octylphenol (OP) and nonylphenol (NP) on decidualization of endometrial stromal cells. Telomerase-immortalized human endometrial stromal cells were cultured for 12 days and decidualization was induced in vitro. The cells were exposed to OP (5 or 10 μM) or NP (5 μM) during in vitro decidualization. The expression levels of decidualization markers were analyzed using qRT-PCR. The amount of prolactin (PRL) and insulin-like growth factor binding protein 1 (IGFBP1) was measured in medium using ELISA. The expression levels of PRL and IGFBP1 were significantly decreased by treatment with OP or NP. The amounts of PRL and IGFBP1 were also significantly decreased by treatment with OP or NP. The patterns of PRL and IGFBP1 secretion into medium were consistent with their expression patterns. The findings indicate that OP or NP may disrupt the decidualization of endometrial stromal cells and eventually affect the reproductive health of women.
本研究探讨了辛基酚（OP）和壬基酚（NP）对子宫内膜基质细胞蜕膜化的影响。永生化的人子宫内膜基质细胞培养 12 天，并在体外诱导蜕膜化。在体外蜕膜化过程中，细胞暴露于 OP（5 或 10 μM）或 NP（5 μM）中。使用 qRT-PCR 分析蜕膜化标记物的表达水平。使用 ELISA 测量培养基中催乳素（PRL）和胰岛素样生长因子结合蛋白 1（IGFBP1）的量。OP 或 NP 处理显著降低了 PRL 和 IGFBP1 的表达水平。OP 或 NP 处理也显著降低了 PRL 和 IGFBP1 的量。PRL 和 IGFBP1 分泌到培养基中的模式与其表达模式一致。这些发现表明，OP 或 NP 可能破坏子宫内膜基质细胞的蜕膜化，最终影响女性的生殖健康。
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