Biological Sciences Department, California Polytechnic State University, San Luis Obispo, CA, 93407, USA.
Biological Sciences Department, California Polytechnic State University, San Luis Obispo, CA, 93407, USA.
Mar Environ Res. 2019 Aug;149:137-147. doi: 10.1016/j.marenvres.2019.06.002. Epub 2019 Jun 6.
Responses of marine ectotherms to variable environmental temperature often entails maintanence of cellular homeostasis and physiological function through temperature compensation and physiological changes. We investigated the physiological response to thermal stress by examining proteomic changes in the marine kelp forest gastropod and emerging fisheries species Kellet's whelk (Kelletia kelletii) across a naturally-existing thermal gradient that ranges from a warmer-water site inside the species' native range and extends to the northern, cold-water edge of the range. We hypothesized that abundance of cellular stress response and energy metabolism proteins would increase with decreasing temperature in support of cold-compensation. Our exploratory proteomic analysis of whelk gill tissue (N = 6 whelks) from each of the four California Channel Island sites revealed protein abundance changes related to the cytoskeleton, energy metabolism/oxidative stress, and cell signaling. The changes did not correlate consistently with temperature. Nonetheless, whelks from the coldest island site showed increased abundance of energy metabolism and oxidative stress proteins, possibly suggesting oxidative damage of lipid membranes that is ameliorated by antioxidants and may aid in their cold stress response. Similarly, our exploratory analysis revealed abundances of cell signaling proteins that were higher at the coldest site compared to the warmest site, possibly indicating an importance for cell signaling regulation in relatively cooler environments. This study provides protein targets for future studies related to thermal effects in marine animals and may contribute to understanding the physiological response of marine organisms to future ocean conditions.
海洋外温动物对环境温度变化的反应通常需要通过温度补偿和生理变化来维持细胞内稳态和生理功能。我们通过检查海洋海带林腹足纲动物和新兴渔业物种 Kellet's whelk（Kelletia kelletii）的蛋白质组变化，研究了对热应激的生理反应，该物种的自然存在的温度梯度范围从其本地范围内较温暖的水域到范围的北部冷水边缘。我们假设细胞应激反应和能量代谢蛋白的丰度会随着温度的降低而增加，以支持冷补偿。我们对来自加利福尼亚海峡群岛四个地点的 6 只 whelk 鳃组织进行的探索性蛋白质组分析表明，与细胞骨架、能量代谢/氧化应激和细胞信号转导有关的蛋白质丰度发生了变化。这些变化与温度没有一致的相关性。尽管如此，来自最寒冷岛屿的 whelks 表现出能量代谢和氧化应激蛋白的丰度增加，这可能表明脂质膜的氧化损伤被抗氧化剂减轻，并且可能有助于它们的冷应激反应。同样，我们的探索性分析揭示了细胞信号蛋白的丰度在最寒冷的地点比在最温暖的地点更高，这可能表明细胞信号调节在相对较冷的环境中很重要。这项研究为未来与海洋动物热效应相关的研究提供了蛋白质靶标，并可能有助于理解海洋生物对未来海洋条件的生理反应。
