精油、商业杀菌剂和低氧微环境对博物馆霉菌链格孢熏蒸作用的新见解

New Insight on Fumigation Action of Essential Oil, Commercial Fungicide and Low Oxygen Microenvironment on Museum Mold, Alternaria alternata.

作者信息

Abdelrahman Elamin, Takatori Kosuke, Matsuda Yasunori, Tsukada Masahiko, Kirino Fumiyoshi

机构信息

Graduate School of Conservation and Cultural Properties, Tokyo University of the Arts.

Conservation Center, Grand Egyptian Museum, Giza, Egypt.

出版信息

Biocontrol Sci. 2019;24(2):123-127. doi: 10.4265/bio.24.123.

DOI:10.4265/bio.24.123

PMID:31204357

Abstract

Fumigation has been the most convenient method in the field of pest control in museums. In this study, as fumigants, ethanol 70%, deltamethrin (commercial pesticide (CP) ) , essential oil (EO) from Pinus regida, and low oxygen microenvironment (0.1%, (LOM) ) were tested individually and jointly against museum fungal strain Alternaria alternata. Three concentrations of each CP and EO were chosen for evaluating the individual effect. In the joint action fumigation process, three lower concentrations of CP and EO were tested in LOM. The rate of mycelial growth inhibition at each fumigation process was determined by two steps: 1) directly after the fumigation process and 2) after 7 d of the inoculation of the fumigated spores in new medium and incubating it in normal condition. The results demonstrated that applying of each chemical (CP or EO) in LOM enhanced its fungicidal activity and that effect of EO improved from fungistatic to fungicidal by jointing with LOM.

摘要

熏蒸一直是博物馆害虫防治领域最便捷的方法。在本研究中，作为熏蒸剂，分别对70%乙醇、溴氰菊酯（商业杀虫剂（CP））、红松精油（EO）和低氧微环境（0.1%，（LOM））进行了单独及联合测试，以对抗博物馆真菌菌株链格孢。每种CP和EO选择了三种浓度来评估其单独效果。在联合作用熏蒸过程中，在低氧微环境中测试了三种较低浓度的CP和EO。每个熏蒸过程中菌丝生长抑制率通过两个步骤确定：1）熏蒸过程刚结束后；2）将熏蒸后的孢子接种到新培养基中并在正常条件下培养7天后。结果表明，在低氧微环境中使用每种化学药剂（CP或EO）均可增强其杀菌活性，并且EO与低氧微环境联合使用时，其效果从抑菌变为杀菌。