Department of Food Technology and Food Material Science, Technische Universität Berlin, Berlin, Germany.
Department of Research and Innovation, National Institute of Research and Development for Food Bioresources - IBA Bucharest, Bucharest, Romania.
J Sci Food Agric. 2019 Oct;99(13):5852-5857. doi: 10.1002/jsfa.9858. Epub 2019 Jul 29.
During the industrial production of β-glucan, a protein-rich fraction remains as a by-product. Recovery of this protein as oat protein concentrate (OPC) results in a source of cereal protein for food and improves the overall economy of the process. In this study, a yoghurt-type product is developed by lactic acid fermentation of an OPC suspension after subjection to heat treatment to assure starch gelatinization.
In detail, the process of yoghurt production involved an initial heating step to 90 °C, followed by 24 h fermentation with a starter culture consisting of Lactobacillus delbrueckii subsp. bulgaricus und Streptococcus thermophilus. The resulting yoghurt-type product was mildly sour (pH 4.2) with a certain amount of lactic acid (3.3 ± 0.2 g kg ) and contained 4.9 × 10 cfu g lactobacillus after 24 h fermentation. Scanning electron microscopy revealed a porous network presumably built up from the gelatinized starch fraction containing aggregated structures, between which were assumed to be aggregated oat proteins. Moreover, to a limited extent, proteolysis occurred during fermentation. Thus some of the proteolytic enzymes present in the yoghurt culture cleaved oat protein and released peptides. However, the effect on essential amino acids was small.
The results of this study provide a deeper knowledge into the role of starch and protein in fermented OPC yoghurts. The structure of fermented OPC verifies the applicability of oat protein as an alternative source for yoghurt-type products. © 2019 Society of Chemical Industry.
在β-葡聚糖的工业生产过程中,会产生一种富含蛋白质的副产物。回收这种蛋白质作为燕麦蛋白浓缩物(OPC),可得到一种用于食品的谷物蛋白来源,并提高整个工艺的经济效益。在这项研究中,通过对 OPC 悬浮液进行热处理以确保淀粉糊化,然后进行乳酸发酵,开发出一种酸奶型产品。
详细来说,酸奶生产过程包括初始加热步骤(加热至 90℃),然后用含有德氏乳杆菌保加利亚亚种和嗜热链球菌的发酵剂进行 24 小时发酵。所得酸奶型产品略带酸味(pH 值为 4.2),具有一定量的乳酸(3.3±0.2 g/kg),发酵 24 小时后含有 4.9×109cfu/g 乳酸菌。扫描电子显微镜显示,一个多孔网络可能是由含有聚集结构的糊化淀粉组成的,在这些聚集结构之间,假设是聚集的燕麦蛋白。此外,在发酵过程中发生了一定程度的蛋白水解。因此,酸奶培养物中存在的一些蛋白酶裂解了燕麦蛋白并释放出肽。然而,对必需氨基酸的影响很小。
本研究结果深入了解了淀粉和蛋白质在发酵 OPC 酸奶中的作用。发酵 OPC 的结构验证了燕麦蛋白作为酸奶型产品替代来源的适用性。© 2019 化学工业协会。