抗裸鼠淋巴细胞抗血清的特性

Properties of antisera against lymphocytes of nude mice.

作者信息

Rudnicka W, Długońska H, Zelazowski P, Szewczyk K, Gościcka T

出版信息

Arch Immunol Ther Exp (Warsz). 1978;26(1-6):339-42.

PMID:312069

Abstract

This paper reports results of a study on the activity of rabbit antisera against nu/nu Balb/c lymphocytes in vivo and in vitro. It was found that ALS against nu/nu lymph node cells suppressed the alloantigen reaction and the sRFC or PFC formation for T-dependent (SRBC) and T-independent (LPS) antigens. ALS against nu/nu spleen cells affected only the sRFC and PFC for T-independent antigen. The former serum exhibited a high cytotoxicity for the suspensions enriched or depleted in B cells while the latter one was more cytotoxic for the suspension enriched in B cells. It indicates that ALS anti nu/nu spleen cells is specific for B lymphocytes and ALS anti nu/nu lymph node cells is directed not only to B cells but also to a subpopulation of T lymphocytes. It suggests the existence of a subpopulation of T lymphocytes in nu/nu lymph node cells.

摘要

本文报道了一项关于兔抗血清对裸鼠/裸 Balb/c 淋巴细胞体内和体外活性的研究结果。发现抗裸鼠/裸淋巴结细胞的抗淋巴细胞血清（ALS）抑制了同种抗原反应以及针对 T 依赖（SRBC）和 T 非依赖（LPS）抗原的 sRFC 或 PFC 形成。抗裸鼠/裸脾细胞的 ALS 仅影响针对 T 非依赖抗原的 sRFC 和 PFC。前者血清对富含或耗尽 B 细胞的悬液表现出高细胞毒性，而后者对富含 B 细胞的悬液细胞毒性更强。这表明抗裸鼠/裸脾细胞的 ALS 对 B 淋巴细胞具有特异性，而抗裸鼠/裸淋巴结细胞的 ALS 不仅针对 B 细胞，还针对 T 淋巴细胞的一个亚群。这提示在裸鼠/裸淋巴结细胞中存在 T 淋巴细胞亚群。