Nichols R, Carlson D M, Dixon J E
Biochemistry Department, Purdue University, West Lafayette, IN 47907.
Biochem Biophys Res Commun. 1987 Nov 30;149(1):244-8. doi: 10.1016/0006-291x(87)91631-7.
Ovine and rat salivary gland mRNAs have been prepared and their translation products characterized. A 60 kD translation product from ovine submaxillary and sublingual gland mRNAs is identical in mass to the ovine apomucin. Two additional ovine translation products, 25 and 40 kD, are specific to mucin-producing salivary glands. Four rat mRNA translation products are encoded by mucin-producing salivary glands (38, 44, 67, 69 kD). These polypeptides were not detected in the parotid gland mRNAs, a serous gland. Each of these products has a high level of [3H]serine incorporation, a characteristic of mucins. The nature of these products suggests that they are mucins or mucin-like and that their molecular weights should approximate that of the corresponding apomucins.
已制备绵羊和大鼠唾液腺的mRNA，并对其翻译产物进行了表征。来自绵羊下颌下腺和舌下腺mRNA的一种60kD翻译产物在质量上与绵羊脱辅基粘蛋白相同。另外两种绵羊翻译产物，25kD和40kD，是产生粘蛋白的唾液腺所特有的。四种大鼠mRNA翻译产物由产生粘蛋白的唾液腺编码（38kD、44kD、67kD、69kD）。在浆液性腺腮腺的mRNA中未检测到这些多肽。这些产物中的每一种都有高水平的[3H]丝氨酸掺入，这是粘蛋白的一个特征。这些产物的性质表明它们是粘蛋白或类粘蛋白，并且它们的分子量应接近相应脱辅基粘蛋白的分子量。
