用于检测冷藏肉和牛奶中假单胞菌属的免疫棒比色测定法。
Immunostick Colorimetric Assay for Detection of Pseudomonas spp. in Refrigerated Meat and Milk.
作者信息
Gutiérrez Rosalba, García Teresa, González Isabel, Sanz Bernabé, Hernández Pablo E, Martín Rosario
机构信息
Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
出版信息
J Food Prot. 1997 Aug;60(8):908-911. doi: 10.4315/0362-028X-60.8.908.
Monoclonal antibodies generated against live cells of Pseudomonas fluorescens have been used in an immunostick enzyme-linked immunosorbent assay (ELISA) format for the detection of Pseudomonas spp. in refrigerated meat and milk. The detection threshold for the immunostick ELISA assay developed in this work is 10 CPU cm for meat and 10 CPU ml for milk samples.
针对荧光假单胞菌活细胞产生的单克隆抗体已用于免疫棒酶联免疫吸附测定（ELISA）形式，以检测冷藏肉和牛奶中的假单胞菌属。本研究中开发的免疫棒ELISA测定法的检测阈值为：肉类为10 CPU/cm，牛奶样品为10 CPU/ml。
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