通过间接酶联免疫吸附测定法对冷藏肉中假单胞菌属进行单克隆抗体检测。
Monoclonal antibody detection of Pseudomonas spp. in refrigerated meat by an indirect ELISA.
作者信息
Gutierrez R, García T, González I, Sanz B, Hernández P E, Martín R
机构信息
Departamento de Nutrición y Bromatologia III, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
出版信息
Lett Appl Microbiol. 1997 Jan;24(1):5-8. doi: 10.1046/j.1472-765x.1997.00329.x.
Abstract
Monoclonal antibodies generated against live cells of Pseudomonas fluorescens have been used in an indirect ELISA format for the detection of Pseudomonas spp. in refrigerated meat. The detection threshold for the ELISA assay developed in this work was 10(4) cfu cm-2.
摘要
针对荧光假单胞菌活细胞产生的单克隆抗体已用于间接酶联免疫吸附测定（ELISA）形式，以检测冷藏肉中的假单胞菌属。这项工作中开发的ELISA检测方法的检测阈值为10(4) 菌落形成单位每平方厘米（cfu cm-2）。
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