miR-25 通过 CDH1 促进人非小细胞肺癌的侵袭。
miR-25 promotes invasion of human non-small cell lung cancer via CDH1.
机构信息
a Department of Thoracic surgery , the central hospital of Linyi , Yishui , Shandong , China.
b Department of Clinical Lab , Qingdao Women and Children's Hospital , Qingdao , Shandong , China.
出版信息
Bioengineered. 2019 Dec;10(1):271-281. doi: 10.1080/21655979.2019.1632668.
MicroRNA-25 (miR-25) has been reported to be overexpressed in numerous human tumors and plays a key role in tumor promotor. However, there are few reports about miR-25 expression and function in non-small cell lung cancer (NSCLC). In this study, we investigated the biological role of miR-25 in NSCLC and its underlying molecular mechanisms. We found that the upregulation of miR-25 was correlated with lymph node metastasis and TNM stage in 113 NSCLC patients. Moreover, the up-regulation of miR-25 was associated with poor survival of NSCLC patients and might be used as an independent prognostic factor. Moreover, forced expression of miR-25 enhanced H358 and Calu-1 cell migration and invasion, but not apoptosis and proliferation . Elevation of invasion and metastasis by miR-25 directly and significantly correlated with inactivation of CDH1 expression. Therefore, patients with up-regulated miR-25 are prone to lymph node metastasis and thus have a poor prognosis.
miR-25（微 RNA-25）在许多人类肿瘤中过表达，在肿瘤促进中起关键作用。然而，关于 miR-25 在非小细胞肺癌（NSCLC）中的表达和功能的报道很少。在这项研究中，我们研究了 miR-25 在 NSCLC 中的生物学作用及其潜在的分子机制。我们发现，113 例 NSCLC 患者中 miR-25 的上调与淋巴结转移和 TNM 分期相关。此外，miR-25 的上调与 NSCLC 患者的不良预后相关，可能作为独立的预后因素。此外，miR-25 的强制表达增强了 H358 和 Calu-1 细胞的迁移和侵袭，但不影响凋亡和增殖。miR-25 通过直接显著降低 CDH1 表达来促进侵袭和转移。因此，上调 miR-25 的患者易发生淋巴结转移，因此预后不良。
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