Department of Biology, University of Bergen, PO Box 7803, Bergen 5020, Norway.
Centre for Geobiology, University of Bergen.
FEMS Microbiol Ecol. 2019 Jul 1;95(7). doi: 10.1093/femsec/fiz091.
Environmental conditions influence species composition, including the microbial communities that associate with benthic organisms such as corals. In this study we identified and compared bacteria that associate with three common deep-water corals, Lophelia pertusa, Madrepora oculata and Paragorgia arborea, from a reef habitat on the mid-Norwegian shelf. The 16S rRNA gene amplicon sequencing data obtained revealed that >50% of sequences were represented by only five operational taxonomic units. Three were host-specific and unclassified below class level, belonging to Alphaproteobacteria with affiliation to members of the Rhizobiales order (L. pertusa), Flavobacteria affiliated with members of the Elisabethkingia genus (M. oculata) and Mollicutes sequences affiliated with the Mycoplasma genus (P. arborea). In addition, gammaproteobacterial sequences within the genera Sulfitobacter and Oleispira were found across all three deep-water coral taxa. Although highly abundant in the coral microbiomes, these sequences accounted for <0.1% of the surrounding bacterioplankton, supporting specific relationships. We combined this information with previous studies, undertaking a meta-data analysis of 165 widespread samples across coral hosts and habitats. Patterns in bacterial diversity indicated enrichment of distinct uncultured species in coral microbiomes that differed among deep (>200 m), mesophotic (30-200 m) and shallow (<30 m) reefs.
环境条件会影响物种组成，包括与底栖生物（如珊瑚）相关的微生物群落。在这项研究中，我们从挪威中部大陆架的一个珊瑚礁生境中鉴定并比较了三种常见深海珊瑚（Lophelia pertusa、Madrepora oculata 和 Paragorgia arborea）所关联的细菌。获得的 16S rRNA 基因扩增子测序数据显示，超过 50%的序列仅由 5 个操作分类单元代表。其中 3 个是宿主特异性的，在科级以下未分类，属于α变形菌，与根瘤菌目（L. pertusa）的成员有关；与伊丽莎白菌属（M. oculata）成员有关的黄杆菌；与支原体属（P. arborea）有关的柔膜体纲序列。此外，在所有三种深海珊瑚类群中还发现了γ变形菌门的 Sulfitobacter 和 Oleispira 属序列。尽管这些序列在珊瑚微生物组中高度丰富，但它们仅占周围浮游细菌的<0.1%，支持特定的关系。我们将这些信息与之前的研究结合起来，对 165 个广泛分布的珊瑚宿主和生境样本进行了元数据分析。细菌多样性的模式表明，在珊瑚微生物组中，独特的未培养物种丰富度不同，这些物种在深海（>200 米）、中光（30-200 米）和浅海（<30 米）珊瑚礁中存在差异。
