Division of Cellular and Molecular Biology, Department of Biology, Faculty of Science, University of Isfahan, Isfahan, 81746-73441, Iran.
J Cancer Res Clin Oncol. 2019 Aug;145(8):1987-1998. doi: 10.1007/s00432-019-02949-5. Epub 2019 Jun 18.
Based on the poor prognosis of drug resistance in pediatric acute lymphoblastic leukemia (ALL) and adverse effects of chemotherapy, this study was aimed to evaluate the effect of several herbal extracts on leukemic cells.
Two subtypes of T- and B-ALL cell lines, followed by ALL primary cells were treated with cinnamon, ginger, and green tea extracts, alone or in combination with methotrexate (MTX). Possible apoptosis was investigated using Annexin-V/PI double staining. Real-time PCR was applied to evaluate the expression levels of related ABC transporters upon combination therapy.
The ICs for cinnamon, ginger and green tea extracts on ALL cell lines were 300 μg/ml, 167 μg/ml and 70 μg/ml, respectively. Surprisingly, the methotrexate (MTX)-resistant sub-line showed more sensitivity to ginger. Combined treatment with ginger and MTX showed synergistic effects on CCRF-CEM, Nalm-6 and ALL primary cells. It was shown that ginger does not impair the high expression levels of ABCA2 or ABCA3 transporter genes in the ALL malignant cells, suggesting other molecular pathways involved in its anticancer potential.
To the best of our knowledge, this is the first study that reveals the antileukemic effect of ginger extract on both, pediatric ALL cell lines and primary cells.
鉴于小儿急性淋巴细胞白血病（ALL）耐药预后较差且化疗存在不良反应，本研究旨在评估几种草药提取物对白血病细胞的作用。
采用肉桂、生姜和绿茶提取物单独或联合甲氨蝶呤（MTX）处理 T-和 B-ALL 细胞系及 ALL 原代细胞，采用 Annexin-V/PI 双重染色法研究可能的细胞凋亡情况。采用实时 PCR 评估联合治疗后相关 ABC 转运体的表达水平。
肉桂、生姜和绿茶提取物对 ALL 细胞系的 IC 分别为 300μg/ml、167μg/ml 和 70μg/ml。令人惊讶的是，MTX 耐药亚系对生姜表现出更高的敏感性。生姜与 MTX 联合治疗对 CCRF-CEM、Nalm-6 和 ALL 原代细胞具有协同作用。结果表明，生姜不会削弱 ALL 恶性细胞中 ABCA2 或 ABCA3 转运体基因的高表达水平，这表明其抗癌潜能涉及其他分子途径。
据我们所知，这是首次研究生姜提取物对小儿 ALL 细胞系和原代细胞均具有抗白血病作用。
