日本毛茛（Ranunculus japonicus）环氧醇合酶 RjEAS（CYP74A88）的克隆与催化特性研究。
Epoxyalcohol Synthase RjEAS (CYP74A88) from the Japanese Buttercup (Ranunculus japonicus): Cloning and Characterization of Catalytic Properties.
机构信息
Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center of Russian Academy of Sciences, Kazan, Tatarstan, 420111, Russia.
出版信息
Biochemistry (Mosc). 2019 Feb;84(2):171-180. doi: 10.1134/S0006297919020081.
Cytochromes P450 of the CYP74 family play a key role in the lipoxygenase cascade generating oxylipins (products of polyunsaturated fatty acid oxidation). The CYP74 family includes allene oxide synthases, hydroperoxide lyases, divinyl ether synthases, and epoxyalcohol synthases. In this work, we cloned the CYP74A88 gene from the Japanese buttercup (Ranunculus japonicus) and studied the properties of the encoded recombinant protein. The CYP74A88 enzyme specifically converts linoleic acid 9- and 13-hydroperoxides to oxiranyl carbinols 9,10-epoxy-11-hydroxy-12-octadecenoic acid and 11-hydroxy-12,13-epoxy-9-octadecenoic acid, respectively, which was confirmed by GC-MS analysis and kinetic studies. Therefore, the CYP74A88 enzyme is a specific epoxyalcohol synthase.
细胞色素 P450 的 CYP74 家族在脂氧合酶级联反应中发挥关键作用，生成氧化脂类（多不饱和脂肪酸氧化产物）。CYP74 家族包括丙二烯氧化物合酶、过氧化物裂解酶、二乙烯醚合酶和环氧化醇合酶。在这项工作中，我们从日本毛茛（Ranunculus japonicus）中克隆了 CYP74A88 基因，并研究了编码重组蛋白的性质。CYP74A88 酶特异性地将亚油酸 9-和 13-过氧化物转化为环氧化醇 9,10-环氧-11-羟基-12-十八烯酸和 11-羟基-12,13-环氧-9-十八烯酸，这通过 GC-MS 分析和动力学研究得到了证实。因此，CYP74A88 酶是一种特异性的环氧化醇合酶。
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