Gao Liping, Shi Tianming, Wang Zhenhua, Lv Jingjing, Schmull Sebastian, Sun Hong
Department of Physiology, Xuzhou Medical University Xuzhou, Jiangsu Province, China.
National Demonstration Center for Experiment Basic Medical Science Education, Xuzhou Medical University Xuzhou, Jiangsu Province, China.
Am J Transl Res. 2019 May 15;11(5):3018-3028. eCollection 2019.
Vascular patches are commonly applied in tissue repair and reconstruction in congenital cardiac surgery. However, the currently available patch materials are inappropriate to be used in the pediatric population due to their lack of supporting tissue growth potential. In our study an active patch material was developed by seeding pediatric patient's bone marrow stem cells on a decellularized aortic extracellular matrix (ECM) scaffold. The patch was then implanted to repair abdominal aorta defects of nude rats. Two months after implantation, tissue remodeling, vascular cell regeneration, and cellular integration were investigated using histology and fluorescent staining. Histology demonstrated infiltration of host cells and formation of organized cell layers as well as intact collagen and elastic fibers inside the patch material. Immunofluorescence indicated regeneration of endothelial and smooth muscle cells. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) identified multiple vascularization-promoting components and growth factors in decellularized aortic ECM scaffold. These results demonstrated growth potential and suitability of human derived tissue-engineered patch for vascular reconstruction, and thus, it might be considered in the future as treatment option in pediatric patients.
血管补片常用于先天性心脏手术中的组织修复和重建。然而，由于目前可用的补片材料缺乏支持组织生长的潜力，因此不适用于儿科患者。在我们的研究中，通过将儿科患者的骨髓干细胞接种在脱细胞主动脉细胞外基质（ECM）支架上，开发出了一种活性补片材料。然后将该补片植入裸鼠以修复腹主动脉缺损。植入两个月后，使用组织学和荧光染色研究组织重塑、血管细胞再生和细胞整合情况。组织学显示宿主细胞浸润、有组织的细胞层形成以及补片材料内完整的胶原纤维和弹性纤维。免疫荧光表明内皮细胞和平滑肌细胞再生。液相色谱-串联质谱（LC-MS/MS）鉴定出脱细胞主动脉ECM支架中有多种促进血管生成的成分和生长因子。这些结果证明了人源组织工程补片用于血管重建的生长潜力和适用性，因此，未来可能会被视为儿科患者的一种治疗选择。
