从印度南部一家三级保健医院的肠道外感染分离出 CTX-M 型超广谱β-内酰胺酶。
CTX-M type extended-spectrum β-lactamase in isolated from extra-intestinal infections in a tertiary care hospital in south India.
机构信息
Department of Microbiology, The Madras Medical Mission, Chennai, India.
Division of Infectious Diseases, Nitte University Centre for Science Education & Research, Mangaluru, India.
出版信息
Indian J Med Res. 2019 Feb;149(2):281-284. doi: 10.4103/ijmr.IJMR_2099_17.
BACKGROUND & OBJECTIVES: Infections caused by extended-spectrum β-lactamase (ESBL)-producing Escherichia coli carrying bla genes have been spreading globally, but there are geographical variations in the type of blagenes prevalent and there are scanty data from India. This study was conducted to determine the CTX-M type ESBLs in E. coli isolates obtained from clinical specimens from patients with extra-intestinal infections attending a tertiary care hospital in south India.
METHODS
ESBL-producing E. coli isolated from patients with extra-intestinal infections were subjected to PCR using CTX-M group-specific primers. From a representative isolate, full-length CTX-M-15 gene was amplified and sequenced. An internal fragment of this gene was sequenced in 10 representative isolates.
RESULTS
Of the 300 isolates of E. coli tested, 88 per cent carried CTX-M genes and blawas the most dominant gene present in 90 per cent of the positive isolates. Most (91%) of the isolates positive for bla were sensitive to meropenem.
INTERPRETATION & CONCLUSIONS: Our findings showed bla to be the dominant gene. Based on the data on antimicrobial susceptibility, cefoperazone-sulbactum could be an antimicrobial of choice.
背景与目的
产超广谱β-内酰胺酶（ESBL）的大肠杆菌携带 bla 基因引起的感染在全球范围内传播，但bla 基因流行的类型存在地域差异，而且来自印度的数据很少。本研究旨在确定从印度南部一家三级保健医院就诊的肠外感染患者的临床标本中分离出的大肠埃希菌中 CTX-M 型 ESBL。
方法
对肠外感染患者分离的产 ESBL 大肠杆菌进行 CTX-M 组特异性引物 PCR。从代表性分离株中扩增并测序全长 CTX-M-15 基因。在 10 个代表性分离株中对该基因的内部片段进行测序。
结果
在 300 株大肠埃希菌分离株中，88%携带 CTX-M 基因，bla 是 90%阳性分离株中最主要的基因。bla 阳性的大多数（91%）分离株对美罗培南敏感。
结论与解释
我们的研究结果显示 bla 是主要基因。根据抗生素敏感性数据，头孢哌酮-舒巴坦可能是一种可供选择的抗生素。
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