Gill H K, Liew F Y
Eur J Immunol. 1979 Feb;9(2):101-6. doi: 10.1002/eji.1830090202.
Mice injected subcutaneously with 1 x 10(8) sheep red blood cells (SRBC) developed high levels of delayed-type hypersensitivity (DTH) to SRBC 4-8 days after injection. Such DTH was suppressed when 100 microgram lipopolysaccharide (LPS) was injected intravenously 1-2 days before or at the time of SRBC injection. This suppression of DTH was transferable by spleen, lymph node, thymus and bone marrow cells to sensitized or normal syngeneic recipients, but could not be transferred by serum. Suppressor cells were not induced by LPS alone or SRBC alone, and they were antigen-specific since DTH to chicken red blood cells was not affected. The suppressor cells appeared in the spleen in optimum number 3-4 days after induction. They were theta-negative and Ig-positive as judged by antiserum plus complement treatment and by Ig rosette separation. Attempts to obtain soluble suppressor factor from the suppressor cells by sonication or in vitro incubation were unsuccessful. Mitomycin C treatment of the suppressor cells completely abolished the suppressor activity. Thus, LPS, in conjunction with antigen, appears to induce a population of specific suppressor B cells which are capable of regulating T cell function.
皮下注射1×10⁸个绵羊红细胞（SRBC）的小鼠，在注射后4 - 8天对SRBC产生高水平的迟发型超敏反应（DTH）。当在SRBC注射前1 - 2天或注射时静脉注射100微克脂多糖（LPS）时，这种DTH受到抑制。这种DTH的抑制可通过脾脏、淋巴结、胸腺和骨髓细胞转移给致敏或正常的同基因受体，但不能通过血清转移。单独的LPS或单独的SRBC不会诱导抑制细胞，并且它们具有抗原特异性，因为对鸡红细胞的DTH不受影响。诱导后3 - 4天，抑制细胞在脾脏中数量达到最佳。通过抗血清加补体处理和Ig花环分离判断，它们为θ阴性和Ig阳性。通过超声处理或体外培养从抑制细胞中获得可溶性抑制因子的尝试均未成功。丝裂霉素C处理抑制细胞完全消除了抑制活性。因此，LPS与抗原一起似乎诱导了一群能够调节T细胞功能的特异性抑制性B细胞。
