日本马色拉菌是自由活动的金头狮狨（Leontopithecus chrysomelas - Kuhl，1820）皮肤微生物组的一部分。

Malassezia japonica is part of the cutaneous microbiome of free-ranging golden-headed lion tamarins (Leontopithecus chrysomelas - Kuhl, 1820).

机构信息

Laboratory of Molecular and Cellular Biology, Paulista University - UNIP, São Paulo, Brazil.

Laboratory of Wildlife Comparative Pathology, School of Veterinary Medicine and Animal Sciences, University of São Paulo - USP, São Paulo, Brazil.

出版信息

Med Mycol. 2020 Jan 1;58(1):133-136. doi: 10.1093/mmy/myz017.

DOI:10.1093/mmy/myz017

PMID:31220312

Abstract

We investigated Malassezia spp. in external ear canal and haircoat of free-ranging golden-headed lion tamarins (Leontopithecus chrysomelas). A total of 199 animals were restrained, and 597 clinical samples were collected. After the amplification of the 26S ribosomal gene by polymerase chain reaction (PCR), the RFLP technique was performed. Two additional PCR protocols were performed in 10 randomly selected strains. Malassezia sp. was isolated in 38.2% (76/199) of the animals and 14.6% (87/597) of the samples; all strains were lipodependent. The 10 sequenced strains showed a high identity with Malassezia japonica, species described in man, but not in animals, so far.

摘要

我们调查了自由放养的金头狮狨（Leontopithecus chrysomelas）的外耳道和毛发中的马拉色菌属。共约束了 199 只动物，并采集了 597 份临床样本。通过聚合酶链反应（PCR）扩增 26S 核糖体基因后，进行了 RFLP 技术。在 10 株随机选择的菌株中进行了另外两个 PCR 方案。马拉色菌属在 38.2%（199/597）的动物和 14.6%（76/199）的样本中被分离出来；所有菌株均依赖于脂肪。测序的 10 株菌株与迄今为止在人类中描述的马拉色菌属（Malassezia japonica）具有高度的同一性，但在动物中尚未发现。