Institute of Epidemiology and Preventive Medicine, College of Public Health, National Taiwan University, Taipei, Taiwan.
Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.
Sci Rep. 2019 Jun 20;9(1):8947. doi: 10.1038/s41598-019-45040-2.
Memory T helper (Th) and regulatory T (Treg) cells play key roles in asthma. Certain sialyl carbohydrate determinants for selectins profoundly affect the migratory properties of memory Th cells, and the suppressive function of Treg cells. Previous studies have shown that the proportion of CCR4 memory Th cells expressing sialyl 6-sulfo Lewis X (Le) is elevated in asthma patients. We aim to investigate the roles of different sialyl glycans on T cell subsets in asthma. Using flow cytometry, we assessed the expression of three sialyl glycans, sialyl 6-sulfo Le, cyclic sialyl 6-sulfo Le, and sialyl Le on memory Th and Treg cells, in the peripheral blood of asthmatic children. We also assessed the relationships between glycan-expressing cell percentages and asthma clinical parameters. Compared with controls, asthmatic children showed higher proportions of memory Th cells expressing sialyl Le and sialyl 6-sulfo Le. The proportions of memory Th cells with sialyl 6-sulfo Le and cyclic sialyl 6-sulfo Le expression in asthmatic children correlated with absolute eosinophil count and IgE level, respectively. Children with moderate-to-severe asthma had lower numbers of sialyl Le positive Treg cells. Our study suggests that sialyl glycans on T cells may play important roles in the pathogenesis of asthma.
记忆 T 辅助细胞（Th）和调节性 T（Treg）细胞在哮喘中发挥关键作用。某些选择素的唾液酸化碳水化合物决定簇深刻影响记忆 Th 细胞的迁移特性和 Treg 细胞的抑制功能。先前的研究表明，哮喘患者中表达唾液酸化 6-硫酸路易斯 X（Le）的 CCR4 记忆 Th 细胞的比例升高。我们旨在研究不同唾液酸化糖在哮喘中 T 细胞亚群中的作用。我们使用流式细胞术评估了哮喘儿童外周血中记忆 Th 和 Treg 细胞上三种唾液酸化糖，即唾液酸化 6-硫酸 Le、环唾液酸化 6-硫酸 Le 和唾液酸化 Le 的表达。我们还评估了糖表达细胞百分比与哮喘临床参数之间的关系。与对照组相比，哮喘儿童表达唾液酸化 Le 和唾液酸化 6-硫酸 Le 的记忆 Th 细胞比例更高。哮喘儿童中表达唾液酸化 6-硫酸 Le 和环唾液酸化 6-硫酸 Le 的记忆 Th 细胞比例与嗜酸性粒细胞绝对计数和 IgE 水平分别相关。中重度哮喘儿童的 sialyl Le 阳性 Treg 细胞数量较少。我们的研究表明，T 细胞上的唾液酸化糖可能在哮喘的发病机制中发挥重要作用。
