Saponari Maria, Zicca Stefania, Loconsole Giuliana, Navarro Beatriz, Di Serio Francesco
Istituto per la Protezione Sostenibile delle Piante, Consiglio Nazionale delle Ricerche, Bari, Italy.
Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università di Bari "Aldo Moro", Bari, Italy.
Methods Mol Biol. 2019;2015:67-78. doi: 10.1007/978-1-4939-9558-5_6.
Citrus can host a number of important vector- and graft-transmissible pathogens which cause severe diseases. Citrus disease management and clean stock programs require pathogen detection systems which must be economical and sensitive to maintain a healthy citrus industry. Rapid diagnostic tests for simultaneous detection of major graft-transmissible disease agents enable reduction of cost and time. The genetic and biological features of viruses and viroids can vary according to the strains/variants, with severe and mild strains described within the same species. The use of diagnostic tests that can allow to selectively discriminate severe strain(s) is a powerful tool to intercept the most harmful strains and to reduce the need for biological indexing. Moreover a combination of these detection methods will facilitate the studies on the interactions between CTV and viroids, a research topic only partially explored so far.
柑橘可携带多种重要的由媒介传播和嫁接传播的病原体，这些病原体会引发严重疾病。柑橘病害管理和无病苗木计划需要病原体检测系统，该系统必须经济且灵敏，以维持柑橘产业的健康发展。同时检测主要嫁接传播病害病原体的快速诊断测试能够降低成本和时间。病毒和类病毒的遗传和生物学特性会因菌株/变体而异，同一物种内既有严重菌株也有温和菌株。使用能够选择性区分严重菌株的诊断测试是拦截最有害菌株并减少生物学索引需求的有力工具。此外，这些检测方法的组合将有助于开展关于柑橘衰退病毒（CTV）和类病毒之间相互作用的研究，这是一个迄今为止仅得到部分探索的研究课题。
