Muthabathula Prajna, Suneetha Saragadam, Grace Ratna
Department of Botany, Andhra University, Visakhapatnam, Andhra Pradesh, India.
2Department of Botany, Dr. V. S. Krishna Government Degree College, Visakhapatnam, Andhra Pradesh, India.
Bioinformation. 2018 Dec 28;14(9):580-586. doi: 10.6026/97320630014580. eCollection 2018.
Codon usage bias analysis allows in identifying the factors that are influencing and contributing to shape the evolution of the organisms. Therefore, it is of interest to analyze 10363 gene sequences from . The GC content with 51.50% is higher than the AT content (48.50%) in B. bassiana. The fungal nuclear genes tend to be GC rich and predominantly G/C ending. Codon usage bias exhibited by is based on the Relative Synonymous Codon Usage (RSCU) values of 61 sense codons, of which 28 codons are with RSCU value larger than 1. Other factors like Nucleotide composition, mutational pressure and selection also has a role in shaping the codon usage bias. We identified 24 optimal codons that end with G or C. Correlation analysis suggests existence of translational efficiency of amino acids. Based on the GC3s distribution evolution of the genes is by the contribution of mutation pressure. ENC may be the major factor in shaping the codon usage bias. This study provides insights into the compositional selection pressure of the genes in .
密码子使用偏好性分析有助于识别影响和促成生物体进化的因素。因此，分析来自球孢白僵菌的10363个基因序列很有意义。球孢白僵菌的GC含量为51.50%，高于AT含量（48.50%）。真菌核基因倾向于富含GC且主要以G/C结尾。基于61个有义密码子的相对同义密码子使用（RSCU）值显示出密码子使用偏好性，其中28个密码子的RSCU值大于1。核苷酸组成、突变压力和选择等其他因素在塑造密码子使用偏好性方面也发挥作用。我们鉴定出24个以G或C结尾的最优密码子。相关性分析表明氨基酸存在翻译效率。基于GC3s分布，基因的进化受到突变压力的影响。有效密码子数（ENC）可能是塑造密码子使用偏好性的主要因素。本研究为球孢白僵菌中基因的组成选择压力提供了见解。
